{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:44:17Z","timestamp":1791567857494,"version":"4.3.4"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":1731,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Corneal blindness is the fourth leading cause of blindness in the world. Current treatment is allogenic corneal transplantation, which is limited by shortage of donors and immunological rejection. Skin-derived precursors (SKPs) are postnatal stem cells, which are self-renewing, multipotent precursors that can be isolated and expanded from the dermis. Facial skin may therefore be an accessible autologous source of neural crest derived cells. SKPs were isolated from facial skin of Wnt1-Cre\/Floxed EGFP mouse. After inducing differentiation with medium containing retinoic acid and GSK 3-\u03b2 inhibitor, SKPs formed polygonal corneal endothelial-like cells (sTECE). Expression of major corneal endothelial markers were confirmed by Reverse transcription polymerase chain reaction (RT-PCR) and quantitative Real time polymerase chain reaction (qRT-PCR). Western blots confirmed the expression of Na, K-ATPase protein, the major functional marker of corneal endothelial cells. Immunohistochemistry revealed the expression of zonular occludens-1 and Na, K-ATPase in cell-cell junctions. In vitro functional analysis of Na, K-ATPase pump activity revealed that sTECE had significantly high pump function compared to SKPs or control 3T3 cells. Moreover, sTECE transplanted into a rabbit model of bullous keratopathy successfully maintained corneal thickness and transparency. Furthermore, we successfully induced corneal endothelial-like cells from human SKPs, and showed that transplanted corneas also maintained corneal transparency and thickness. Our findings suggest that SKPs may be used as a source of autologous cells for the treatment of corneal endothelial disease.<\/jats:p>","DOI":"10.1002\/sctm.16-0162","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T09:33:43Z","timestamp":1486373623000},"page":"788-798","source":"Crossref","is-referenced-by-count":44,"title":["Skin-Derived Precursors as a Source of Progenitors for Corneal Endothelial Regeneration"],"prefix":"10.1093","volume":"6","author":[{"given":"Emi","family":"Inagaki","sequence":"first","affiliation":[{"name":"a Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Hatou","sequence":"additional","affiliation":[{"name":"a Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Higa","sequence":"additional","affiliation":[{"name":"b Department of Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Ichikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Yoshida","sequence":"additional","affiliation":[{"name":"a Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinsuke","family":"Shibata","sequence":"additional","affiliation":[{"name":"c Department of Physiology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Okano","sequence":"additional","affiliation":[{"name":"c Department of Physiology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Tsubota","sequence":"additional","affiliation":[{"name":"a Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeto","family":"Shimmura","sequence":"additional","affiliation":[{"name":"a Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"2022022809404616900_R1","first-page":"229","article-title":"Clinical estimation of corneal endothelial pump function","volume":"96","author":"Bourne","year":"1998","journal-title":"Trans Am Ophthalmol Soc."},{"key":"2022022809404616900_R2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S1350-9462(02)00065-4","article-title":"Proliferative capacity of the corneal endothelium","volume":"22","author":"Joyce","year":"2003","journal-title":"Prog Retin Eye Res"},{"key":"2022022809404616900_R3","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1097\/00006324-200110000-00010","article-title":"A population study of the normal cornea using an in vivo, slit-scanning confocal microscope","volume":"78","author":"Hollingsworth","year":"2001","journal-title":"Optom Vis Sci"},{"key":"2022022809404616900_R4","doi-asserted-by":"crossref","first-page":"7888","DOI":"10.1167\/iovs.11-8040","article-title":"Mathematical projection model of visual loss due to fuchs corneal dystrophy","volume":"52","author":"Hatou","year":"2011","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"2022022809404616900_R5","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1097\/TP.0b013e3182111f01","article-title":"Human corneal endothelial cell expansion for corneal endothelium transplantation: An overview","volume":"91","author":"Peh","year":"2011","journal-title":"Transplantation"},{"key":"2022022809404616900_R6","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.1016\/j.ophtha.2013.05.025","article-title":"Global prevalence of vision impairment and blindness: magnitude and temporal trends, 1990-2010","volume":"120","author":"Stevens","year":"2013","journal-title":"Ophthalmology"},{"key":"2022022809404616900_R7","doi-asserted-by":"crossref","first-page":"254","DOI":"10.3109\/08820539409060024","article-title":"Glaucoma after penetrating keratoplasty","volume":"9","author":"Doyle","year":"1994","journal-title":"Semin Ophthalmol"},{"key":"2022022809404616900_R8","doi-asserted-by":"crossref","first-page":"2609","DOI":"10.1016\/j.transproceed.2007.08.020","article-title":"Epidemiology and risk factors for corneal graft rejection","volume":"39","author":"Sellami","year":"2007","journal-title":"Transplant Proc"},{"key":"2022022809404616900_R9","first-page":"828","article-title":"Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt\/beta-catenin signaling. 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