{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:58:40Z","timestamp":1789750720505,"version":"4.0.1"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":1750,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Hepatocellular carcinoma (HCC) is believed to originate from cancer stem cells (CSCs). While epithelial cell adhesion molecule (EpCAM) is a marker of normal hepatic stem cells (HSCs), EpCAM+ cells from HCC behave like CSCs. Since HCC mostly develops on a cirrhotic background, we sought to determine whether CSC-like EpCAM+ cells exist in patients with advanced cirrhosis. Both flow cytometry and immunohistochemistry showed that frequency of EpCAM+ cells in advanced cirrhosis was increased as compared to control. To determine whether increased EpCAM population in advanced cirrhosis harbors any CSC-like cells, we compared molecular and functional features of EpCAM+ cells from advanced cirrhosis (Ep+CIR; n\u2009=\u200920) with EpCAM+ cells from both HCC (Ep+HCC; n\u2009=\u200920) and noncancerous\/noncirrhotic (control) (Ep+NSC; n\u2009=\u20097) liver tissues. Ep+CIRs displayed similarity with Ep+HCC cells including upregulated expression of stemness and Notch pathway genes, enhanced self-renewal in serial spheroid assay and generation of subcutaneous tumors in nonobese diabetic\/severe combined immunodeficiency mice. Moreover, transcriptome and miRNome of Ep+CIRs appeared closer to that of Ep+HCC cells than Ep+NSCs. Interestingly, more than 50% micro RNAs (miRNAs) and transcripts specifically expressed in Ep+HCCs were also expressed in Ep+CIRs. However, none of Ep+NSC specific miRNAs and only 7% Ep+NSC specific transcripts were expressed in Ep+CIRs. Further, according to gene expression and in vitro Wnt inhibition analysis, autocrine Wnt signaling appeared to be a distinct feature of Ep+CIR and Ep+HCC cells, which was absent from Ep+NSCs. EpCAM+ cells in advanced cirrhosis possibly include a population of CSC-like cells which can be explored for early diagnosis of HCC development.<\/jats:p>","DOI":"10.1002\/sctm.16-0248","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T08:56:43Z","timestamp":1486544203000},"page":"807-818","source":"Crossref","is-referenced-by-count":37,"title":["EpCAM+ Liver Cancer Stem-Like Cells Exhibiting Autocrine Wnt Signaling Potentially Originate in Cirrhotic Patients"],"prefix":"10.1093","volume":"6","author":[{"given":"Ritu","family":"Khosla","sequence":"first","affiliation":[{"name":"a Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Rastogi","sequence":"additional","affiliation":[{"name":"b Department of Pathology, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayatri","family":"Ramakrishna","sequence":"additional","affiliation":[{"name":"a Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viniyendra","family":"Pamecha","sequence":"additional","affiliation":[{"name":"c Department of Liver Transplant and Hepato Pancreato Biliary Surgery, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"d Stem Cell Biology Laboratory, National Institute of Immunology, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madavan","family":"Vasudevan","sequence":"additional","affiliation":[{"name":"e Bionivid Technology Pvt Ltd, Bangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiv Kumar","family":"Sarin","sequence":"additional","affiliation":[{"name":"f Department of Hepatology, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirupma","family":"Trehanpati","sequence":"additional","affiliation":[{"name":"a Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,1,18]]},"reference":[{"key":"2022022809411031800_R1","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1002\/hep.27996","article-title":"RBMY, a novel inhibitor of glycogen synthase kinase 3beta, increases tumor stemness and predicts poor prognosis of hepatocellular 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