{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:53:35Z","timestamp":1790524415509,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T00:00:00Z","timestamp":1638835200000},"content-version":"vor","delay-in-days":1636,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"State Scientific Key Projects for New Drug Research and Development","award":["2011ZX09102-010-04"],"award-info":[{"award-number":["2011ZX09102-010-04"]}]},{"name":"State Scientific Key Projects for New Drug Research and Development","award":["2011ZX09401-027"],"award-info":[{"award-number":["2011ZX09401-027"]}]},{"name":"International Cooperation and Exchange Program","award":["2013DFA30830"],"award-info":[{"award-number":["2013DFA30830"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The ex vivo generation of human red blood cells on a large scale from hematopoietic stem and progenitor cells has been considered as a potential method to overcome blood supply shortages. Here, we report that functional human erythrocytes can be efficiently produced from cord blood (CB) CD34+ cells using a bottle turning device culture system. Safety and efficiency studies were performed in murine and nonhuman primate (NHP) models. With the selected optimized culture conditions, one human CB CD34+ cell could be induced ex vivo to produce up to 200 million erythrocytes with a purity of 90.1%\u2009\u00b1\u20096.2% and 50%\u2009\u00b1\u20095.7% (mean\u2009\u00b1\u2009SD) for CD235a+ cells and enucleated cells, respectively. The yield of erythrocytes from one CB unit (5 million CD34+ cells) could be, in theory, equivalent to 500 blood transfusion units in clinical application. Moreover, induced human erythrocytes had normal hemoglobin content and could continue to undergo terminal maturation in the murine xenotransplantation model. In NHP model, xenotransplantation of induced human erythrocytes enhanced hematological recovery and ameliorated the hypoxia situation in the primates with hemorrhagic anemia. These findings suggested that the ex vivo-generated erythrocytes could be an alternative blood source for traditional transfusion products in the clinic.<\/jats:p>","DOI":"10.1002\/sctm.17-0057","type":"journal-article","created":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T11:38:05Z","timestamp":1497526685000},"page":"1698-1709","source":"Crossref","is-referenced-by-count":52,"title":["Large-Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34+ Cells"],"prefix":"10.1093","volume":"6","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"},{"name":"b Biopharmagen Corp, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Wang","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Shen","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Guan","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tian","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, 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Stony Brook, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Dai","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"},{"name":"e Department of Environment Medicine, New York University Langone Medical center, Tuxedo, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Jiang","sequence":"additional","affiliation":[{"name":"a Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, China"},{"name":"b Biopharmagen Corp, Suzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,6,15]]},"reference":[{"key":"2022022809523114000_R1","volume-title":"Global Database on Blood Safety: Summary Report","author":"World Health 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