{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:50:25Z","timestamp":1791985825524,"version":"4.4.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":955,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,4,19]],"date-time":"2019-04-19T00:00:00Z","timestamp":1555632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,4,19]],"date-time":"2019-04-19T00:00:00Z","timestamp":1555632000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"University of Iowa Institute for Vision Research Endowment","award":["NEI R01-EY026547"],"award-info":[{"award-number":["NEI R01-EY026547"]}]},{"name":"University of Iowa Institute for Vision Research Endowment","award":["P30 EY025580"],"award-info":[{"award-number":["P30 EY025580"]}]},{"name":"Lidsky\/Zawadzki\/McGuinn\/Cohen Families"},{"DOI":"10.13039\/100010849","name":"Hope for Vision","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018133","name":"VitreoRetinal Surgery Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018133","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Howard F. Ruby Endowment for Human Retinal Engineering"},{"DOI":"10.13039\/100010849","name":"Hope For Vision","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010849","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Subretinal delivery of stem cell-derived retinal cells as a strategy to treat retinal degenerative blindness holds great promise. Currently, two clinical trials are underway in which human fetal retinal progenitor cells (RPCs) are being delivered to patients by intravitreal or subretinal injection to preserve or restore vision, respectively. With the advent of the induced pluripotent stem cell (iPSC), and in turn three-dimensional derivation of retinal tissue, it is now possible to generate autologous RPCs for cell replacement. The purpose of this study was to evaluate the effect of commonly used cell isolation and surgical manipulation strategies on donor cell viability. iPSC-RPCs were subjected to various conditions, including different dissociation and isolation methods, injection cannula sizes, and preinjection storage temperatures and times. The effects of commonly used surgical techniques on both host and donor cell viability were evaluated in Yucatan mini-pigs (n\u2009=\u200961 eyes). We found a significant increase in cell viability when papain was used for RPC isolation. In addition, a significant decrease in cell viability was detected when using the 41G cannula compared with 31G and at storage times of 4 hours compared with 30\u2009minutes. Although 96.4% of all eyes demonstrated spontaneous retinal reattachment following injection, retinal pigment epithelium (RPE) abnormalities were seen more frequently in eyes receiving injections via a 31G cannula; interestingly, eyes that received cell suspensions were relatively protected against such RPE changes. These findings indicate that optimization of donor cell isolation and delivery parameters should be considered when developing a subretinal cell replacement strategy. Stem Cells Translational Medicine \u00a02019;8:797\u2013809<\/jats:p>\n               <jats:sec>\n                  <jats:title\/>\n               <\/jats:sec>","DOI":"10.1002\/sctm.18-0210","type":"journal-article","created":{"date-parts":[[2019,4,20]],"date-time":"2019-04-20T06:38:39Z","timestamp":1555742319000},"page":"797-809","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Optimizing Donor Cellular Dissociation and Subretinal Injection Parameters for Stem Cell-Based Treatments"],"prefix":"10.1093","volume":"8","author":[{"given":"Brittni A.","family":"Scruggs","sequence":"first","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Jiao","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathryn M.","family":"Cranston","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Kaalberg","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"b Department of Biostatistics College of Public Health, University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Russell","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke A.","family":"Wiley","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Mullins","sequence":"additional","affiliation":[{"name":"a University of Iowa Institute for Vision Research and the Department of Ophthalmology and Visual Sciences University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin 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