{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:32:00Z","timestamp":1790073120333,"version":"4.0.1"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":986,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIH\/NIGMS","doi-asserted-by":"publisher","award":["1R01GM110628-01"],"award-info":[{"award-number":["1R01GM110628-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK090971"],"award-info":[{"award-number":["DK090971"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1R01GM110628\u201001"],"award-info":[{"award-number":["1R01GM110628\u201001"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK090971"],"award-info":[{"award-number":["DK090971"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bacterial and fungal infections are a major cause of morbidity and mortality in neutropenic patients. Donor-derived neutrophil transfusions have been used for prophylaxis or treatment for infection in neutropenic patients. However, the short half-life and the limited availability of large numbers of donor-derived neutrophils for transfusion remain a significant hurdle in the implementation of neutrophil transfusion therapy. Here, we investigate the in vitro and in vivo activity of neutrophils generated from human induced pluripotent stem cells (iPSC), a potentially unlimited resource to produce neutrophils for transfusion. Phenotypic analysis of iPSC-derived neutrophils reveal reactive oxygen species production at similar or slightly higher than normal peripheral blood neutrophils, but have an \u223c50%\u201370% reduced Escherichia coli phagocytosis and phorbol 12-myristate 13-acetate induced formation of neutrophil extracellular traps (NET). Signaling of granulocytic precursors identified impaired AKT activation, but not ERK or STAT3, in agonist-stimulated iPSC-derived neutrophils. Expression of a constitutively activated AKT in iPSC-derived neutrophils restores most phagocytic activity and NET formation. In a model of bacterial induced peritonitis in immunodeficient mice, iPSC-derived neutrophils, with or without corrected AKT activation, migrate similarly to the peritoneal fluid as peripheral blood neutrophils, whereas the expression of activated AKT significantly improves their phagocytic activity in vivo. Stem Cells Translational Medicine \u00a02019;8:557\u2013567<\/jats:p>\n               <jats:sec>\n                  <jats:title\/>\n               <\/jats:sec>","DOI":"10.1002\/sctm.18-0255","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T05:22:25Z","timestamp":1550812945000},"page":"557-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Neutrophils Derived from Genetically Modified Human Induced Pluripotent Stem Cells Circulate and Phagocytose Bacteria In Vivo"],"prefix":"10.1093","volume":"8","author":[{"given":"Lisa R.","family":"Trump","sequence":"first","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh C.","family":"Nayak","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek K.","family":"Singh","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"},{"name":"b Hoxworth Blood Center University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Emberesh","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley M.","family":"Wellendorf","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn M.","family":"Lutzko","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"},{"name":"b Hoxworth Blood Center University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Cancelas","sequence":"additional","affiliation":[{"name":"a Division of Experimental Hematology and Cancer Biology Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA"},{"name":"b Hoxworth Blood Center University of Cincinnati College of Medicine, Cincinnati, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"2022022810310230900_sct312467-bib-0001","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/ajh.23107","article-title":"Guidelines for pediatric management of severe chronic neutropenia","volume":"87","author":"Dale","year":"2012","journal-title":"Am J Hematol"},{"key":"2022022810310230900_sct312467-bib-0002","doi-asserted-by":"crossref","first-page":"1826","DOI":"10.3324\/haematol.2013.091025","article-title":"European guidelines for empirical antibacterial therapy for febrile neutropenic patients in the era of growing resistance: Summary of the 2011 4th European Conference on Infections in Leukemia","volume":"98","author":"Averbuch","year":"2013","journal-title":"Haematologica"},{"key":"2022022810310230900_sct312467-bib-0003","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1007\/s00277-014-2086-0","article-title":"Management of sepsis in neutropenic patients: 2014 updated guidelines from the Infectious Diseases Working Party of the German Society of Hematology and Medical Oncology (AGIHO)","volume":"93","author":"Penack","year":"2014","journal-title":"Ann Hematol"},{"key":"2022022810310230900_sct312467-bib-0004","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/annonc\/mdu192","article-title":"Diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients (allogeneic SCT excluded): Updated guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO)","volume":"26","author":"Maschmeyer","year":"2015","journal-title":"Ann Oncol"},{"key":"2022022810310230900_sct312467-bib-0005","doi-asserted-by":"crossref","first-page":"2258","DOI":"10.1002\/cncr.21847","article-title":"Mortality, morbidity, and cost associated with febrile neutropenia in adult cancer patients","volume":"106","author":"Kuderer","year":"2006","journal-title":"Cancer"},{"key":"2022022810310230900_sct312467-bib-0006","doi-asserted-by":"crossref","first-page":"4628","DOI":"10.1182\/blood-2005-11-4370","article-title":"The incidence of leukemia and mortality from sepsis in patients with severe congenital neutropenia receiving long-term G-CSF therapy","volume":"107","author":"Rosenberg","year":"2006","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0007","doi-asserted-by":"crossref","first-page":"3158","DOI":"10.1200\/JCO.2006.08.8823","article-title":"Impact of primary prophylaxis with granulocyte colony-stimulating factor on febrile neutropenia and mortality in adult cancer patients receiving chemotherapy: A systematic review","volume":"25","author":"Kuderer","year":"2007","journal-title":"J Clin Oncol"},{"key":"2022022810310230900_sct312467-bib-0008","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0022-1759(99)00178-7","article-title":"Clinical and laboratory work-up of patients with neutrophil shortage or dysfunction","volume":"232","author":"Kuijpers","year":"1999","journal-title":"J Immunol Methods"},{"key":"2022022810310230900_sct312467-bib-0009","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1182\/blood.V47.5.869.869","article-title":"Experimental Pseudomonas pneumonia in leukopenic dogs: Comparison of therapy with antibiotics and granulocyte transfusions","volume":"47","author":"Dale","year":"1976","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0010","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3181\/00379727-84-20539","article-title":"Transfusion of separated leukocytes into irradiated dogs with aplastic marrows","volume":"84","author":"Brecher","year":"1953","journal-title":"Proc Soc Exp Biol Med"},{"key":"2022022810310230900_sct312467-bib-0011","doi-asserted-by":"crossref","first-page":"6702","DOI":"10.1182\/blood-2010-09-309864","article-title":"Pretreatment with phosphatase and tensin homolog deleted on chromosome 10 (PTEN) inhibitor SF1670 augments the efficacy of granulocyte transfusion in a clinically relevant mouse model","volume":"117","author":"Li","year":"2011","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0012","first-page":"CD003956","article-title":"Granulocyte transfusions for neonates with confirmed or suspected sepsis and neutropenia","volume":"10","author":"Pammi","year":"2011","journal-title":"Cochrane Database Syst Rev"},{"key":"2022022810310230900_sct312467-bib-0013","first-page":"CD005341","article-title":"Granulocyte transfusions for preventing infections in patients with neutropenia or neutrophil dysfunction","volume":"1","author":"Massey","year":"2009","journal-title":"Cochrane Database Syst Rev"},{"key":"2022022810310230900_sct312467-bib-0014","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1182\/blood-2015-05-645986","article-title":"Efficacy of transfusion with granulocytes from G-CSF\/dexamethasone-treated donors in neutropenic patients with infection","volume":"126","author":"Price","year":"2015","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0015","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nature05934","article-title":"Generation of germline-competent induced pluripotent stem cells","volume":"448","author":"Okita","year":"2007","journal-title":"Nature"},{"key":"2022022810310230900_sct312467-bib-0016","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.stem.2012.05.005","article-title":"Induced pluripotent stem cells: Past, present, and future","volume":"10","author":"Yamanaka","year":"2012","journal-title":"Cell Stem Cell"},{"key":"2022022810310230900_sct312467-bib-0017","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1182\/blood-2013-07-513242","article-title":"Neutropenia-associated ELANE mutations disrupting translation initiation produce novel neutrophil elastase isoforms","volume":"123","author":"Tidwell","year":"2014","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0018","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.stemcr.2015.01.005","article-title":"Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies","volume":"4","author":"Lachmann","year":"2015","journal-title":"Stem Cell Rep"},{"key":"2022022810310230900_sct312467-bib-0019","doi-asserted-by":"crossref","first-page":"3103","DOI":"10.1172\/JCI80924","article-title":"Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells","volume":"125","author":"Nayak","year":"2015","journal-title":"J Clin Invest"},{"key":"2022022810310230900_sct312467-bib-0020","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1172\/JCI38591","article-title":"Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors","volume":"119","author":"Choi","year":"2009","journal-title":"J Clin Invest"},{"key":"2022022810310230900_sct312467-bib-0021","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1038\/nprot.2010.184","article-title":"Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells","volume":"6","author":"Choi","year":"2011","journal-title":"Nat Protoc"},{"key":"2022022810310230900_sct312467-bib-0022","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1089\/scd.2010.0063","article-title":"Direct generation of induced pluripotent stem cells from human nonmobilized blood","volume":"20","author":"Kunisato","year":"2011","journal-title":"Stem Cells Dev"},{"key":"2022022810310230900_sct312467-bib-0023","doi-asserted-by":"crossref","first-page":"e27956","DOI":"10.1371\/journal.pone.0027956","article-title":"Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors","volume":"6","author":"Mack","year":"2011","journal-title":"PLoS One"},{"key":"2022022810310230900_sct312467-bib-0024","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1038\/mt.2010.314","article-title":"Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming","volume":"19","author":"Warlich","year":"2011","journal-title":"Mol Ther"},{"key":"2022022810310230900_sct312467-bib-0025","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.stemcr.2016.05.006","article-title":"Integrated genomic analysis of diverse induced pluripotent stem cells from the progenitor cell biology consortium","volume":"7","author":"Salomonis","year":"2016","journal-title":"Stem Cell Rep"},{"key":"2022022810310230900_sct312467-bib-0026","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1182\/blood-2012-05-429589","article-title":"Steady-state neutrophil homeostasis is dependent on TLR4\/TRIF signaling","volume":"121","author":"Bugl","year":"2013","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0027","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.molimm.2013.11.016","article-title":"MLK3 regulates fMLP-stimulated neutrophil motility","volume":"58","author":"Polesskaya","year":"2014","journal-title":"Mol Immunol"},{"key":"2022022810310230900_sct312467-bib-0028","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.stemcr.2015.01.005","article-title":"Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies","volume":"4","author":"Lachmann","year":"2015","journal-title":"Stem Cell Rep"},{"key":"2022022810310230900_sct312467-bib-0029","first-page":"493","article-title":"Effect of glycosylation of recombinant human granulocytic colony-stimulating factor on expansion cultures of umbilical cord blood CD34+ cells","volume":"84","author":"Querol","year":"1999","journal-title":"Haematologica"},{"key":"2022022810310230900_sct312467-bib-0030","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/imm.12123","article-title":"Kinase AKT controls innate immune cell development and function","volume":"140","author":"Zhang","year":"2013","journal-title":"Immunology"},{"key":"2022022810310230900_sct312467-bib-0031","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ejphar.2009.04.050","article-title":"Inhibition of superoxide anion generation by CHS-111 via blockade of the p21-activated kinase, protein kinase B\/Akt and protein kinase C signaling pathways in rat neutrophils","volume":"615","author":"Chang","year":"2009","journal-title":"Eur J Pharmacol"},{"key":"2022022810310230900_sct312467-bib-0032","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1073\/pnas.0800218105","article-title":"Inositol trisphosphate 3-kinase B (InsP3KB) as a physiological modulator of myelopoiesis","volume":"105","author":"Jia","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022022810310230900_sct312467-bib-0033","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/S0014-5793(01)02669-2","article-title":"p90-RSK and Akt may promote rapid phosphorylation\/inactivation of glycogen synthase kinase 3 in chemoattractant-stimulated neutrophils","volume":"502","author":"De Mesquita","year":"2001","journal-title":"FEBS Lett"},{"key":"2022022810310230900_sct312467-bib-0034","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1182\/blood-2009-11-255323","article-title":"Akt isoforms differentially regulate neutrophil functions","volume":"115","author":"Chen","year":"2010","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0035","doi-asserted-by":"crossref","first-page":"31372","DOI":"10.1074\/jbc.271.49.31372","article-title":"Expression of a constitutively active Akt Ser\/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation","volume":"271","author":"Kohn","year":"1996","journal-title":"J Biol Chem"},{"key":"2022022810310230900_sct312467-bib-0036","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s00281-013-0380-x","article-title":"Modulation of neutrophil NETosis: Interplay between infectious agents and underlying host physiology","volume":"35","author":"Hahn","year":"2013","journal-title":"Semin Immunopathol"},{"key":"2022022810310230900_sct312467-bib-0037","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1097\/00062752-200105000-00006","article-title":"Granulocyte transfusion therapy: Update on potential clinical applications","volume":"8","author":"Hubel","year":"2001","journal-title":"Curr Opin Hematol"},{"key":"2022022810310230900_sct312467-bib-0038","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1046\/j.1537-2995.2002.00249.x","article-title":"Granulocyte transfusion therapy for infections in candidates and recipients of HPC transplantation: A comparative analysis of feasibility and outcome for community donors versus related donors","volume":"42","author":"Hubel","year":"2002","journal-title":"Transfusion"},{"key":"2022022810310230900_sct312467-bib-0039","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/00062752-200105000-00005","article-title":"Safety of granulocyte colony-stimulating factor in normal donors","volume":"8","author":"Gutierrez-Delgado","year":"2001","journal-title":"Curr Opin Hematol"},{"key":"2022022810310230900_sct312467-bib-0040","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1111\/j.1365-2141.2006.06312.x","article-title":"Haematological malignancies developing in previously healthy individuals who received haematopoietic growth factors: Report from the Research on Adverse Drug Events and Reports (RADAR) project","volume":"135","author":"Bennett","year":"2006","journal-title":"Br J Haematol"},{"key":"2022022810310230900_sct312467-bib-0041","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1111\/j.1365-2141.2006.06361.x","article-title":"Possible harmful effects of short course granulocyte colony-stimulating factor in normal donors","volume":"135","author":"Goldman","year":"2006","journal-title":"Br J Haematol"},{"key":"2022022810310230900_sct312467-bib-0042","volume-title":"Granulocytes, Pooled, Buffy Coat Derived, in Platelet Additive Solution and Plamsa Guidelines for the Blood Transfusion Services in the United Kingdom. 8: TSO","year":"2017"},{"key":"2022022810310230900_sct312467-bib-0043","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/trf.12907","article-title":"Spectra optia granulocyte apheresis collections result in higher collection efficiency of viable, functional neutrophils in a randomized, crossover, multicenter trial","volume":"55","author":"Cancelas","year":"2015","journal-title":"Transfusion"},{"key":"2022022810310230900_sct312467-bib-0044","doi-asserted-by":"crossref","first-page":"3535","DOI":"10.1182\/blood-2004-07-2676","article-title":"Single infusion of myeloid progenitors reduces death from Aspergillus fumigatus following chemotherapy-induced neutropenia","volume":"105","author":"BitMansour","year":"2005","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0045","doi-asserted-by":"crossref","first-page":"4660","DOI":"10.1182\/blood-2002-05-1552","article-title":"Myeloid progenitors protect against invasive aspergillosis and Pseudomonas aeruginosa infection following hematopoietic stem cell transplantation","volume":"100","author":"BitMansour","year":"2002","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0046","doi-asserted-by":"crossref","first-page":"7043","DOI":"10.1200\/JCO.2018.36.15_suppl.7043","article-title":"A randomized open label exploratory controlled trial of CLT-008 myeloid progenitor cells (MPC) to decrease infections during induction for AML","volume":"36","author":"Abboud","year":"2018","journal-title":"J Clin Oncol"},{"key":"2022022810310230900_sct312467-bib-0047","volume-title":"Decreased Incedince of Infection, Use of Antibacterials and Days in Hospital after Administration of CLT-008 Myeloid Progenitor Cells to Subjects Receiving AML Induction Therapy: Phase 2 Study Results","author":"Desai","year":"2018"},{"key":"2022022810310230900_sct312467-bib-0048","doi-asserted-by":"crossref","first-page":"3517","DOI":"10.1074\/jbc.M005953200","article-title":"p38 kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophils","volume":"276","author":"Rane","year":"2001","journal-title":"J Biol Chem"},{"key":"2022022810310230900_sct312467-bib-0049","doi-asserted-by":"crossref","first-page":"5302","DOI":"10.4049\/jimmunol.170.10.5302","article-title":"Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils","volume":"170","author":"Chen","year":"2003","journal-title":"J Immunol"},{"key":"2022022810310230900_sct312467-bib-0050","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"2022022810310230900_sct312467-bib-0051","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nm.2184","article-title":"Reciprocal coupling of coagulation and innate immunity via neutrophil serine proteases","volume":"16","author":"Massberg","year":"2010","journal-title":"Nat Med"},{"key":"2022022810310230900_sct312467-bib-0052","doi-asserted-by":"crossref","first-page":"2400","DOI":"10.2741\/3386","article-title":"Regulation of neutrophil apoptosis by modulation of PKB\/Akt activation","volume":"14","author":"Rane","year":"2009","journal-title":"Front Biosci"},{"key":"2022022810310230900_sct312467-bib-0053","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1182\/blood.V87.3.983.bloodjournal873983","article-title":"A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation","volume":"87","author":"Lin","year":"1996","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0054","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1182\/blood-2013-09-526707","article-title":"Akt is essential to induce NADPH-dependent NETosis and to switch the neutrophil death to apoptosis","volume":"123","author":"Douda","year":"2014","journal-title":"Blood"},{"key":"2022022810310230900_sct312467-bib-0055","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/ni.2052","article-title":"Inositol hexakisphosphate kinase 1 regulates neutrophil function in innate immunity by inhibiting phosphatidylinositol-(3,4,5)-trisphosphate signaling","volume":"12","author":"Prasad","year":"2011","journal-title":"Nat Immunol"},{"key":"2022022810310230900_sct312467-bib-0056","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.200602142","article-title":"DOCK2 is a Rac activator that regulates motility and polarity during neutrophil chemotaxis","volume":"174","author":"Kunisaki","year":"2006","journal-title":"J Cell Biol"},{"key":"2022022810310230900_sct312467-bib-0057","doi-asserted-by":"crossref","first-page":"5727","DOI":"10.4049\/jimmunol.172.9.5727","article-title":"Phosphoinositide 3-kinase and Akt occupy central roles in inflammatory responses of Toll-like receptor 2-stimulated neutrophils","volume":"172","author":"Strassheim","year":"2004","journal-title":"J Immunol"},{"key":"2022022810310230900_sct312467-bib-0058","doi-asserted-by":"crossref","first-page":"8064","DOI":"10.4049\/jimmunol.174.12.8064","article-title":"Involvement of SHIP in TLR2-induced neutrophil activation and acute lung injury","volume":"174","author":"Strassheim","year":"2005","journal-title":"J Immunol"},{"key":"2022022810310230900_sct312467-bib-0059","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1165\/rcmb.2006-0457OC","article-title":"Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils","volume":"37","author":"Wong","year":"2007","journal-title":"Am J Respir Cell Mol Biol"}],"container-title":["Stem Cells Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fsctm.18-0255","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/sctm.18-0255","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/sctm.18-0255","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/8\/6\/557\/42630284\/stcltm_8_6_557.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/stcltm\/article-pdf\/8\/6\/557\/42630284\/stcltm_8_6_557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T10:31:57Z","timestamp":1646044317000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/stcltm\/article\/8\/6\/557\/6403906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,21]]},"references-count":59,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,2,21]]},"published-print":{"date-parts":[[2019,6,1]]}},"URL":"https:\/\/doi.org\/10.1002\/sctm.18-0255","relation":{},"ISSN":["2157-6564","2157-6580"],"issn-type":[{"value":"2157-6564","type":"print"},{"value":"2157-6580","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,6,1]]},"published":{"date-parts":[[2019,2,21]]}}}