{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:42:20Z","timestamp":1792478540300,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"23-24","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2P30CA016058"],"award-info":[{"award-number":["2P30CA016058"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R50CA243786"],"award-info":[{"award-number":["R50CA243786"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI165391"],"award-info":[{"award-number":["T32AI165391"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Statistics in Medicine"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A major public health issue is the growing resistance of bacteria to antibiotics. An important part of the needed response is the discovery and development of new antimicrobial strategies. Require the screening of potential new drugs, typically accomplished using high\u2010throughput screening (HTS). Traditionally, HTS is performed by examining one compound per well, but a more efficient strategy pools multiple compounds per well. In this work, we study several recently proposed pooling construction methods, as well as a variety of pooled high\u2010throughput screening analysis methods, in order to provide guidance to practitioners on which methods to use. This is done in the context of an application of the methods to the search for new drugs to combat bacterial infection. We discuss both an extensive pilot study as well as a small screening campaign, and highlight both the successes and challenges of the pooling approach.<\/jats:p>","DOI":"10.1002\/sim.70778","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:56:39Z","timestamp":1792155399000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design and Analysis Strategies for Pooling in High Throughput Screening: Application to the Search for a New Anti\u2010Microbial"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4076-8973","authenticated-orcid":false,"given":"Byran J.","family":"Smucker","sequence":"first","affiliation":[{"name":"Department of Epidemiology &amp; Biostatistics, College of Human Medicine Michigan State University  East Lansing Michigan USA"},{"name":"Henry Ford Health + Michigan State University Health Sciences  Detroit Michigan USA"},{"name":"Department of Public Health Sciences Henry Ford Health  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Brennan","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Henry Ford Health  Detroit Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4635-3503","authenticated-orcid":false,"given":"Emily","family":"Rego","sequence":"additional","affiliation":[{"name":"Department of Microbiology The Ohio State University  Columbus Ohio USA"},{"name":"Department of Microbial Infection and Immunity The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wu","sequence":"additional","affiliation":[{"name":"The Ohio State University Comprehensive Cancer Center\u00a0\u2013\u00a0Arthur G. James Cancer Hospital and Richard J. Solove Research Institute  Columbus Ohio USA"},{"name":"Division of Medicinal Chemistry and Pharmacognosy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4355-243X","authenticated-orcid":false,"given":"Zhihong","family":"Lin","sequence":"additional","affiliation":[{"name":"The Ohio State University Comprehensive Cancer Center\u00a0\u2013\u00a0Arthur G. James Cancer Hospital and Richard J. Solove Research Institute  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4267-7322","authenticated-orcid":false,"given":"Brian M. M.","family":"Ahmer","sequence":"additional","affiliation":[{"name":"Department of Microbiology The Ohio State University  Columbus Ohio USA"},{"name":"Department of Microbial Infection and Immunity The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8251-3579","authenticated-orcid":false,"given":"Blake R.","family":"Peterson","sequence":"additional","affiliation":[{"name":"The Ohio State University Comprehensive Cancer Center\u00a0\u2013\u00a0Arthur G. James Cancer Hospital and Richard J. Solove Research Institute  Columbus Ohio USA"},{"name":"Division of Medicinal Chemistry and Pharmacognosy The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"E.Tacconelli \u201cGlobal priority list of antibiotic\u2010resistant bacteria to guide research discovery and development \u201d(2017)."},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00173-7"},{"key":"e_1_2_11_4_1","unstructured":"T.Coque D. 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