{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:10:06Z","timestamp":1753870206068,"version":"3.41.2"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":697,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mucoviscidose ABCF2"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>A high proportion of patients with Cystic Fibrosis (CF) also present the rare skin disease aquagenic palmoplantar keratoderma. A possible link between this condition and absence of a functional CF Transmembrane conductance Regulator protein in the sweat acinus and collecting duct remains unknown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In-depth characterization of sweat proteome profiles was performed in 25 CF patients compared to 12 healthy controls. A 20 \u03bcL sweat sample was collected after pilocarpine iontophoresis and liquid chromatography tandem mass spectrometry (LC-MS\/MS) proteomic analysis was performed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Sweat proteome profile of CF patients was significantly different from that of healthy subjects with 57 differentially expressed proteins. Cystic Fibrosis sweat proteome was characterized by an increase in 25 proteins including proteases (Kallikrein 7 and 13, Phospholipase B domain containing 1, Cathepsin A L2 and B, Lysosomal Pro-X carboxypeptidase); proinflammatory proteins (Annexin A2, Chitinase-3-like protein 1); cytochrome c and transglutaminases. Thirty-two proteins were downregulated in CF sweat including proteases (Elastase 2), antioxidative protein FAM129 B; membrane-bound transporter SLC6A14 and regulator protein Sodium-hydrogen antiporter 3 regulator 1.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This study is the first to report in-depth characterization of endogenous peptides in CF sweat and could help understand the complex physiology of the sweat gland. The proteome profile highlights the unbalanced proteolytic and proinflammatory activity of sweat in CF. These results also suggest a defect in pathways involved in skin barrier integrity in CF patients. Sweat proteome profile could prove to be a useful tool in the context of personalized medicine in CF.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/ski2.161","type":"journal-article","created":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T07:12:57Z","timestamp":1669446777000},"source":"Crossref","is-referenced-by-count":3,"title":["Proteomic Profiling of Sweat in Patients with Cystic Fibrosis Provides New Insights into Epidermal Homoeostasis"],"prefix":"10.1093","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5466-9373","authenticated-orcid":false,"given":"Matthieu","family":"Cornet","sequence":"first","affiliation":[{"name":"Institut Necker Enfants Malades, INSERM U1151 , Paris ,","place":["France"]},{"name":"Center for Computational Biology, Mines ParisTech, PSL Research University , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao","family":"Nguyen-Khoa","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, INSERM U1151 , Paris ,","place":["France"]},{"name":"Laboratory of Biochemistry, H\u00f4pital Universitaire Necker Enfants Malades AP-HP Centre , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mairead","family":"Kelly-Aubert","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, INSERM U1151 , Paris ,","place":["France"]},{"name":"Universit\u00e9 Paris Cit\u00e9 , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Jung","sequence":"additional","affiliation":[{"name":"Proteomics Platform Necker, Universit\u00e9 Paris Cit\u00e9, Structure F\u00e9d\u00e9rative de Recherche Necker (SFR Necker, INSERM US24\/CNRS UAR3633) , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Chedevergne","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9f\u00e9rence Maladies Rares, Mucoviscidose et Maladies Apparent\u00e9es, H\u00f4pital Necker Enfants Malades AP-HP Centre Paris Cit\u00e9 , Paris ,","place":["France"]},{"name":"European Respiratory Network, ERN Lung , Frankfurt ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muriel","family":"Le Bourgeois","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9f\u00e9rence Maladies Rares, Mucoviscidose et Maladies Apparent\u00e9es, H\u00f4pital Necker Enfants Malades AP-HP Centre Paris Cit\u00e9 , Paris ,","place":["France"]},{"name":"European Respiratory Network, ERN Lung , Frankfurt ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Aoust","sequence":"additional","affiliation":[{"name":"Centre de R\u00e9f\u00e9rence Maladies Rares, Mucoviscidose et Maladies Apparent\u00e9es, H\u00f4pital Necker Enfants Malades AP-HP Centre Paris Cit\u00e9 , Paris ,","place":["France"]},{"name":"European Respiratory Network, ERN Lung , Frankfurt ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00e9vin","family":"Roger","sequence":"additional","affiliation":[{"name":"Proteomics Platform Necker, Universit\u00e9 Paris Cit\u00e9, Structure F\u00e9d\u00e9rative de Recherche Necker (SFR Necker, INSERM US24\/CNRS UAR3633) , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara Ida","family":"Guerrera","sequence":"additional","affiliation":[{"name":"Proteomics Platform Necker, Universit\u00e9 Paris Cit\u00e9, Structure F\u00e9d\u00e9rative de Recherche Necker (SFR Necker, INSERM US24\/CNRS UAR3633) , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Sermet-Gaudelus","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, INSERM U1151 , Paris ,","place":["France"]},{"name":"Universit\u00e9 Paris Cit\u00e9 , Paris ,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"issue":"10059","key":"2025010818114765300_ski2161-bib-0001","doi-asserted-by":"publisher","first-page":"2519","DOI":"10.1016\/s0140-6736(16)00576-6","article-title":"Cystic fibrosis","volume":"388","author":"Elborn","year":"2016","journal-title":"Lancet Lond Engl"},{"issue":"8","key":"2025010818114765300_ski2161-bib-0002","doi-asserted-by":"publisher","first-page":"1717","DOI":"10.1111\/jdv.17312","article-title":"Aquagenic wrinkling of the palms after brief immersion to water test as a screening tool for cystic fibrosis diagnosis","volume":"35","author":"Alexopoulos","year":"2021","journal-title":"J Eur Acad Dermatol Venereol JEADV"},{"issue":"5","key":"2025010818114765300_ski2161-bib-0003","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1111\/j.1525-1470.2011.01708.x","article-title":"High prevalence of aquagenic wrinkling of the palms in patients with cystic fibrosis and association with measurable increases in transepidermal water loss","volume":"29","author":"Arkin","year":"2012","journal-title":"Pediatr Dermatol"},{"issue":"11","key":"2025010818114765300_ski2161-bib-0004","doi-asserted-by":"publisher","first-page":"1296","DOI":"10.1001\/archdermatol.2009.260","article-title":"Aquagenic wrinkling of the palms in cystic fibrosis: comparison with controls and genotype-phenotype correlations","volume":"145","author":"Berk","year":"2009","journal-title":"Arch Dermatol"},{"issue":"2","key":"2025010818114765300_ski2161-bib-0005","doi-asserted-by":"publisher","first-page":"e102","DOI":"10.1016\/j.jcf.2022.01.005","article-title":"Aquagenic wrinkling of the palms in cystic fibrosis patients treated with ivacaftor","volume":"21","author":"Jacobi","year":"2022","journal-title":"J Cyst Fibros Off J Eur Cyst Fibros Soc"},{"issue":"8","key":"2025010818114765300_ski2161-bib-0006","doi-asserted-by":"publisher","DOI":"10.3390\/jpm11080729","article-title":"Sweat chloride testing and nasal potential difference (NPD) are primary outcome parameters in treatment with cystic fibrosis Transmembrane conductance regulator (CFTR) modulators","volume":"11","author":"Sermet-Gaudelus","year":"2021","journal-title":"J Pers Med"},{"issue":"12","key":"2025010818114765300_ski2161-bib-0007","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat Biotechnol"},{"issue":"2","key":"2025010818114765300_ski2161-bib-0008","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00209-2022","article-title":"Reclassifying inconclusive diagnosis for Cystic Fibrosis with new generation sweat test","volume":"60","author":"Nguyen-Khoa","year":"2022","journal-title":"Eur Respir J"},{"issue":"3","key":"2025010818114765300_ski2161-bib-0009","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1183\/09031936.05.10100004","article-title":"The CFTR 3849+10kbC-&gt;T and 2789+5G-&gt;A alleles are associated with a mild CF phenotype","volume":"25","author":"Dugu\u00e9p\u00e9roux","year":"2005","journal-title":"Eur Respir J"},{"key":"2025010818114765300_ski2161-bib-0010","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.jprot.2017.01.005","article-title":"Proteomic and peptidomic analysis of human sweat with emphasis on proteolysis","volume":"155","author":"Yu","year":"2017","journal-title":"J Proteomics"},{"issue":"19","key":"2025010818114765300_ski2161-bib-0011","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910871","article-title":"Characterization of the human eccrine sweat proteome-A focus on the biological variability of individual sweat protein profiles","volume":"22","author":"Burat","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"10","key":"2025010818114765300_ski2161-bib-0012","doi-asserted-by":"publisher","first-page":"2024","DOI":"10.1111\/jdv.13221","article-title":"Highly abundant defense proteins in human sweat as revealed by targeted proteomics and label-free quantification mass spectrometry","volume":"29","author":"Cs\u0151sz","year":"2015","journal-title":"J Eur Acad Dermatol Venereol JEADV"},{"issue":"11","key":"2025010818114765300_ski2161-bib-0013","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203133","article-title":"The proteomic and metabolomic characterization of exercise-induced sweat for human performance monitoring: a pilot investigation","volume":"13","author":"Harshman","year":"2018","journal-title":"PLoS One"},{"issue":"6","key":"2025010818114765300_ski2161-bib-0014","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201800010","article-title":"Eccrine sweat as a biofluid for profiling immune biomarkers","volume":"12","author":"Katchman","year":"2018","journal-title":"Proteomics Clin Appl"},{"issue":"4","key":"2025010818114765300_ski2161-bib-0015","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1021\/pr2007957","article-title":"Proteomic analysis of eccrine sweat: implications for the discovery of schizophrenia biomarker proteins","volume":"11","author":"Raiszadeh","year":"2012","journal-title":"J Proteome Res"},{"issue":"5","key":"2025010818114765300_ski2161-bib-0016","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1002\/prca.201500071","article-title":"Proteomic profiling of eccrine sweat reveals its potential as a diagnostic biofluid for active tuberculosis","volume":"10","author":"Adewole","year":"2016","journal-title":"Proteomics Clin Appl"},{"issue":"9","key":"2025010818114765300_ski2161-bib-0017","doi-asserted-by":"publisher","first-page":"5406","DOI":"10.1074\/jbc.m504670200","article-title":"Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L","volume":"281","author":"Baechle","year":"2006","journal-title":"J Biol Chem"},{"issue":"5","key":"2025010818114765300_ski2161-bib-0018","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1046\/j.1523-1747.2002.19507.x","article-title":"Cathelicidin anti-microbial peptide expression in sweat, an innate defense system for the skin","volume":"119","author":"Murakami","year":"2002","journal-title":"J Invest Dermatol"},{"issue":"4","key":"2025010818114765300_ski2161-bib-0019","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1111\/j.1600-0625.2010.01218.x","article-title":"An antimicrobial protein, lactoferrin exists in the sweat: proteomic analysis of sweat","volume":"20","author":"Park","year":"2011","journal-title":"Exp Dermatol"},{"issue":"12","key":"2025010818114765300_ski2161-bib-0020","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1038\/ni732","article-title":"Dermcidin: a novel human antibiotic peptide secreted by sweat glands","volume":"2","author":"Schittek","year":"2001","journal-title":"Nat Immunol"},{"issue":"5","key":"2025010818114765300_ski2161-bib-0021","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1089\/dia.2011.0262","article-title":"Correlation between sweat glucose and blood glucose in subjects with diabetes","volume":"14","author":"Moyer","year":"2012","journal-title":"Diabetes Technol Ther"},{"issue":"3","key":"2025010818114765300_ski2161-bib-0022","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1080\/23328940.2019.1632145","article-title":"Physiology of sweat gland function: the roles of sweating and sweat composition in human health","volume":"6","author":"Baker","year":"2019","journal-title":"Temp Austin Tex"},{"issue":"1","key":"2025010818114765300_ski2161-bib-0023","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006566","article-title":"KLK5 and KLK7 ablation fully rescues lethality of Netherton syndrome-like phenotype","volume":"13","author":"Kasparek","year":"2017","journal-title":"PLoS Genet"},{"issue":"6","key":"2025010818114765300_ski2161-bib-0024","doi-asserted-by":"publisher","DOI":"10.3390\/cells9061499","article-title":"Annexin A2 in inflammation and host defense","volume":"9","author":"Dallacasagrande","year":"2020","journal-title":"Cells"},{"issue":"11","key":"2025010818114765300_ski2161-bib-0025","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1111\/cea.13478","article-title":"Chitinase 3-like 1 drives allergic skin inflammation via Th2 immunity and M2 macrophage activation","volume":"49","author":"Kwak","year":"2019","journal-title":"Clin Exp Allergy J Br Soc Allergy Clin Immunol"},{"issue":"5","key":"2025010818114765300_ski2161-bib-0026","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.jcf.2015.12.020","article-title":"YKL-40 as marker of severe lung disease in cystic fibrosis patients","volume":"15","author":"Leonardi","year":"2016","journal-title":"J Cyst Fibros Off J Eur Cyst Fibros Soc"},{"issue":"6","key":"2025010818114765300_ski2161-bib-0027","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/35014038","article-title":"Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor","volume":"2","author":"Burns","year":"2000","journal-title":"Nat Cell Biol"},{"key":"2025010818114765300_ski2161-bib-0028","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.ebiom.2019.06.022","article-title":"FAM129B, an antioxidative protein, reduces chemosensitivity by competing with Nrf2 for Keap1 binding","volume":"45","author":"Cheng","year":"2019","journal-title":"EBioMedicine"},{"issue":"3","key":"2025010818114765300_ski2161-bib-0029","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1172\/jci41440","article-title":"Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing","volume":"120","author":"Bonnart","year":"2010","journal-title":"J Clin Invest"},{"issue":"Pt 9","key":"2025010818114765300_ski2161-bib-0030","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1242\/jcs.033969","article-title":"Filaggrin in the frontline: role in skin barrier function and disease","volume":"122","author":"Sandilands","year":"2009","journal-title":"J Cell Sci"},{"issue":"4","key":"2025010818114765300_ski2161-bib-0031","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/ng1767","article-title":"Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis","volume":"38","author":"Palmer","year":"2006","journal-title":"Nat Genet"},{"issue":"1","key":"2025010818114765300_ski2161-bib-0032","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.jaci.2006.05.004","article-title":"Loss-of-function variations within the filaggrin gene predispose for atopic dermatitis with allergic sensitizations","volume":"118","author":"Weidinger","year":"2006","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"2025010818114765300_ski2161-bib-0033","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1083\/jcb.200303036","article-title":"Defining desmosomal plakophilin-3 interactions","volume":"161","author":"Bonn\u00e9","year":"2003","journal-title":"J Cell Biol"},{"issue":"3","key":"2025010818114765300_ski2161-bib-0034","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/j.1365-2133.2012.11003.x","article-title":"Aquagenic (pseudo) keratoderma: a clinical series with new pathological insights","volume":"167","author":"Rongioletti","year":"2012","journal-title":"Br J Dermatol"},{"issue":"1","key":"2025010818114765300_ski2161-bib-0035","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1111\/j.1749-6632.2009.04046.x","article-title":"The role of the NHERF family of PDZ scaffolding proteins in the regulation of salt and water transport","volume":"1165","author":"Seidler","year":"2009","journal-title":"Ann N Y Acad Sci"},{"issue":"17","key":"2025010818114765300_ski2161-bib-0036","doi-asserted-by":"publisher","first-page":"3311","DOI":"10.1007\/s00018-020-03487-x","article-title":"Update on SLC6A14 in lung and gastrointestinal physiology and physiopathology: focus on cystic fibrosis","volume":"77","author":"Ruffin","year":"2020","journal-title":"Cell Mol Life Sci CMLS"}],"container-title":["Skin Health and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ski2.161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ski2.161","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/skinhd\/article-pdf\/3\/1\/ski2.161\/59935103\/skinhd_3_1_e161.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/skinhd\/article-pdf\/3\/1\/ski2.161\/59935103\/skinhd_3_1_e161.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T18:11:55Z","timestamp":1736359915000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/skinhd\/article\/doi\/10.1002\/ski2.161\/7755599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,25]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2,1]]}},"URL":"https:\/\/doi.org\/10.1002\/ski2.161","relation":{},"ISSN":["2690-442X"],"issn-type":[{"type":"electronic","value":"2690-442X"}],"subject":[],"published-other":{"date-parts":[[2023,2]]},"published":{"date-parts":[[2022,11,25]]},"article-number":"ski2.161"}}