{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:38:07Z","timestamp":1791517087460,"version":"4.3.4"},"reference-count":101,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemistrySelect"],"published-print":{"date-parts":[[2022,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The emergence of the novel coronavirus (SARS\u2010CoV\u20102) in December 2019 has generated a devastating global consequence which makes the development of a rapidly deployable, effective and safe vaccine candidate an imminent global health priority. The design of most vaccine candidates has been directed at the induction of antibody responses against the trimeric spike glycoprotein of SARS\u2010CoV\u20102, a class I fusion protein that aids ACE2 (angiotensin\u2010converting enzyme 2) receptor binding. A variety of formulations and vaccinology approaches are being pursued for targeting the spike glycoprotein, including simian and human replication\u2010defective adenoviral vaccines, subunit protein vaccines, nucleic acid vaccines and whole\u2010inactivated SARS\u2010CoV\u20102. Here, we directed a reverse vaccinology approach towards the design of a nucleic acid (mRNA\u2010based) vaccine candidate. The \u201cYLQPRTFLL\u201d peptide sequence (position 269\u2013277) which was predicted to be a B cell epitope and likewise a strong binder of the HLA*A\u20100201 was selected for the design of the vaccine candidate, having satisfied series of antigenicity assessments. Through the codon optimization protocol, the nucleotide sequence for the vaccine candidate design was generated and targeted at the human toll\u2010like receptor 7 (TLR7). Bioinformatics analyses showed that the sequence \u201cUACCUGCAGCCGCGUACCUUCCUGCUG\u201d exhibited a strong affinity and likewise was bound to a stable cavity in the TLR7 pocket. This study is therefore expected to contribute to the research efforts directed at securing definitive preventive measures against the SARS\u2010CoV\u20102 infection.<\/jats:p>","DOI":"10.1002\/slct.202103903","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T07:41:01Z","timestamp":1645083661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Identification of a Potential mRNA\u2010based Vaccine Candidate against the SARS\u2010CoV\u20102 Spike Glycoprotein: A Reverse Vaccinology Approach"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8988-8154","authenticated-orcid":false,"given":"Olanrewaju Ayodeji","family":"Durojaye","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Membraneless Organelle and Cellular Dynamics Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China  Hefei Anhui 230027 China"},{"name":"School of Life Sciences University of Science and Technology of China  Hefei Anhui 230027 China"},{"name":"Department of Chemical Sciences Coal City University, Emene  Enugu State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divine Mensah","family":"Sedzro","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Membraneless Organelle and Cellular Dynamics Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China  Hefei Anhui 230027 China"},{"name":"School of Life Sciences University of Science and Technology of China  Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukhtar Oluwaseun","family":"Idris","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Science and Technology of China  Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeeb Abiodun","family":"Yekeen","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Science and Technology of China  Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeola Abraham","family":"Fadahunsi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Science and Technology of China  Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwaseun Suleiman","family":"Alakanse","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Science and Technology of China  Hefei Anhui 230027 China"},{"name":"Department of Biochemistry Faculty of Life Sciences University of Ilorin  Ilorin Kwara State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,17]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030747"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"N. Zhu D. Zhang W. Wang X. Li B. Yang J. Song X. Zhao B. Huang W. Shi R. Lu A novel coronavirus from patients with pneumonia in China 2019. N. Engl. J. Med.2020.","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51230"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_2_9_8_1","author":"Hoffmann M.","year":"2020","journal-title":"BioRxiv"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2019.08.002"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-019-0233-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1690918"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1546298"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2017.1330236"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2017.5"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.243"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.022"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229159"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3080"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2943"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14138"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01167.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2020.100673"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00623-2"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa892"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv639"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-7580-4-2"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-4528-2"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-131"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.1.1"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx346"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21078"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","unstructured":"S. Saha G.\u2005P.\u2005S. Raghava International Conference on Artificial Immune Systems 2004 p 197.","DOI":"10.1007\/978-3-540-30220-9_16"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-4"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-016-0669-1"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4315"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp363"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky356"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct300854w"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky357"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2020.06.006"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321-017-0246-7"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21214"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.6.2195"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4655(95)00042-E"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.464397"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/8.2.127"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki376"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-285"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg272"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky300"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki375"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1479"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx407"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.9b00332"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00204a009"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0902-646"},{"issue":"4","key":"e_1_2_9_59_1","first-page":"701","volume":"42","author":"MIu L.","year":"2008","journal-title":"Mol. Biol."},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060707"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.109843"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphereDirect.00554-17"},{"key":"e_1_2_9_63_1","author":"Adasme M. F.","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2035389"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00083-06"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2622-0"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2024671"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2028436"},{"key":"e_1_2_9_69_1","unstructured":"F. Polack S. Thomas N. Kitchin Safety and efficacy of the BNT162b2 Covid-19 vaccine.N. Engl. J. Med.https:\/\/doiorg\/10.1056\/NEJMoa2034577. ."},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-082119-124838"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.10.2357"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00197-7"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-007-0004-5"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43042-019-0045-y"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02442.x"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00149-7"},{"key":"e_1_2_9_77_1","first-page":"17","volume":"19","author":"Langeveld J. P.","year":"2001","journal-title":"Vaccine"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0141-9838.2004.00728.x"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.01.019"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.12.010"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2020.107380"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00024"},{"issue":"4","key":"e_1_2_9_83_1","first-page":"201","volume":"5","author":"Bayrami V.","year":"2016","journal-title":"Mol. Biol. Res. Commun."},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2017.1322579"},{"key":"e_1_2_9_85_1","volume":"372","author":"Challen R.","year":"2021","journal-title":"BMJ"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2013.07.006"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2014.02.005"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2014.10.003"},{"key":"e_1_2_9_89_1","first-page":"1","volume":"9","author":"De Ruyck J.","year":"2016","journal-title":"Adv. Appl. Bioinform. Chem."},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci5006004"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2017.1355245"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(76)90184-7"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01311-z"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.04.023"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00594"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix592"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-chembioeng-061010-114133"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.024"},{"key":"e_1_2_9_99_1","first-page":"93","author":"Avci-Adali M.","year":"2014","journal-title":"J. Vis. Exp."},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324198"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1097\/CJI.0b013e3181f7dbe8"}],"container-title":["ChemistrySelect"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/slct.202103903","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/slct.202103903","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/slct.202103903","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T10:35:07Z","timestamp":1761474907000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/slct.202103903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,17]]},"references-count":101,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,2,18]]}},"alternative-id":["10.1002\/slct.202103903"],"URL":"https:\/\/doi.org\/10.1002\/slct.202103903","archive":["Portico"],"relation":{},"ISSN":["2365-6549","2365-6549"],"issn-type":[{"value":"2365-6549","type":"print"},{"value":"2365-6549","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,17]]},"assertion":[{"value":"2021-11-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202103903"}}