{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:41:37Z","timestamp":1791247297539,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"37","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"EU PINET network","award":["FP7-PEOPLE-2010-ITN-264864"],"award-info":[{"award-number":["FP7-PEOPLE-2010-ITN-264864"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Small"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>\n                    The preparation of ultrasmall and rigid platforms (USRPs) that are covalently coupled to macrocycle\u2010based, calcium\u2010responsive\/smart contrast agents (SCAs), and the initial in vitro and in vivo validation of the resulting nanosized probes (SCA\u2010USRPs) by means of magnetic resonance imaging (MRI) is reported. The synthetic procedure is robust, allowing preparation of the SCA\u2010USRPs on a multigram scale. The resulting platforms display the desired MRI activity\u2014i.e., longitudinal relaxivity increases almost twice at 7 T magnetic field strength upon saturation with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Cell viability is probed with the MTT assay using HEK\u2010293 cells, which show good tolerance for lower contrast agent concentrations over longer periods of time. On intravenous administration of SCA\u2010USRPs in living mice, MRI studies indicate their rapid accumulation in the renal pelvis and parenchyma. Importantly, the MRI signal increases in both kidney compartments when CaCl\n                    <jats:sub>2<\/jats:sub>\n                    is also administrated. Laser\u2010induced breakdown spectroscopy experiments confirm accumulation of SCA\u2010USRPs in the renal cortex. To the best of our knowledge, these are the first studies which demonstrate calcium\u2010sensitive MRI signal changes in vivo. Continuing contrast agent and MRI protocol optimizations should lead to wider application of these responsive probes and development of superior functional methods for monitoring calcium\u2010dependent physiological and pathological processes in a dynamic manner.\n                  <\/jats:p>","DOI":"10.1002\/smll.201500312","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T17:21:22Z","timestamp":1436894482000},"page":"4900-4909","source":"Crossref","is-referenced-by-count":49,"title":["Ultrasmall Nanoplatforms as Calcium\u2010Responsive Contrast Agents for Magnetic Resonance Imaging"],"prefix":"10.1002","volume":"11","author":[{"given":"Albert","family":"Moussaron","sequence":"first","affiliation":[{"name":"Laboratoire MATEIS INSA de Lyon 69621 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandip","family":"Vibhute","sequence":"additional","affiliation":[{"name":"Department for Physiology of Cognitive Processes Max Planck Institute for Biological Cybernetics 72076 T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Bianchi","sequence":"additional","affiliation":[{"name":"CRMSB UMR 5536 Universit\u00e9 Bordeaux 33076 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serhat","family":"G\u00fcnd\u00fcz","sequence":"additional","affiliation":[{"name":"MR Neuroimaging Agents Group Max Planck Institute for Biological Cybernetics Spemannstr. 41 72076 T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shady","family":"Kotb","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re UMR CNRS 5306 \u2013 Universit\u00e9 Lyon 1 69622 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Sancey","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re UMR CNRS 5306 \u2013 Universit\u00e9 Lyon 1 69622 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Motto\u2010Ros","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re UMR CNRS 5306 \u2013 Universit\u00e9 Lyon 1 69622 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Rizzitelli","sequence":"additional","affiliation":[{"name":"CRMSB UMR 5536 Universit\u00e9 Bordeaux 33076 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Cr\u00e9millieux","sequence":"additional","affiliation":[{"name":"CRMSB UMR 5536 Universit\u00e9 Bordeaux 33076 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Lux","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re UMR CNRS 5306 \u2013 Universit\u00e9 Lyon 1 69622 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikos K.","family":"Logothetis","sequence":"additional","affiliation":[{"name":"Department for Physiology of Cognitive Processes Max Planck Institute for Biological Cybernetics 72076 T\u00fcbingen Germany"},{"name":"Department of Imaging Science and Biomedical Engineering University of Manchester Manchester M13 9PT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Tillement","sequence":"additional","affiliation":[{"name":"Institut Lumi\u00e8re Mati\u00e8re UMR CNRS 5306 \u2013 Universit\u00e9 Lyon 1 69622 Villeurbanne Cedex France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goran","family":"Angelovski","sequence":"additional","affiliation":[{"name":"MR Neuroimaging Agents Group Max Planck Institute for Biological Cybernetics Spemannstr. 41 72076 T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,14]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmmi.265"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118503652"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/73780"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1093\/oso\/9780195151718.001.0001","volume-title":"Ions in the Brain : Normal Function, Seizures, and Strokes","author":"Somjen G. 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