{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:52:15Z","timestamp":1790787135175,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"33","license":[{"start":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T00:00:00Z","timestamp":1430956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Small"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>Type 2 diabetes mellitus is characterized by the pathological deposition of fibrillized protein, known as amyloids. It is thought that oligomers and\/or amyloid fibrils formed from human islet amyloid polypeptide (hIAPP or amylin) cause cell death by membrane damage. The molecular structure of hIAPP amyloid fibrils is dominated by \u03b2\u2010sheet structure, as probed with conventional infrared and Raman vibrational spectroscopy. However, with these techniques it is not possible to distinguish between the core and the surface structure of the fibrils. Since the fibril surface crucially affects amyloid toxicity, it is essential to know its structure. Here the surface molecular structure and amino acid residue composition of hIAPP fibrils are specifically probed with nanoscale resolution using tip\u2010enhanced Raman spectroscopy (TERS). The fibril surface mainly contains unordered or \u03b1\u2010helical structures, in contrast to the \u03b2\u2010sheet\u2010rich core. This experimentally validates recent models of hIAPP amyloids based on NMR measurements. Spatial mapping of the surface structure reveals a highly heterogeneous surface structure. Finally, TERS can probe fibrils formed on a lipid interface, which is more representative of amyloids in vivo.<\/jats:p>","DOI":"10.1002\/smll.201500562","type":"journal-article","created":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T16:41:25Z","timestamp":1431016885000},"page":"4131-4139","source":"Crossref","is-referenced-by-count":79,"title":["Nanoscale Heterogeneity of the Molecular Structure of Individual hIAPP Amyloid Fibrils Revealed with Tip\u2010Enhanced Raman Spectroscopy"],"prefix":"10.1002","volume":"11","author":[{"given":"Corianne C.","family":"vandenAkker","sequence":"first","affiliation":[{"name":"FOM Institute AMOLF  Science Park 104 1098 XG Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Deckert\u2010Gaudig","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Photonic Technology  Albert\u2010Einstein\u2010Stra\u00dfe 9 07745 Jena 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