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The first reaction involves the adenosine triphosphate (ATP)\u2010dependent conversion of GMP to guanosine diphosphate (GDP) by guanylate kinase, which warrants substrate specificity. The reaction products ADP and GDPare co\u2010substrates for the enzymatic conversion of phosphoenolpyruvate to pyruvate in a second reaction mediated by pyruvate kinase. Pyruvate in turn is the co\u2010substrate for lactate dehydrogenase that generates lactate <jats:italic>via<\/jats:italic> oxidation of nicotinamide adenine dinucleotide (reduced form) NADH to NAD<jats:sup>+<\/jats:sup>. This third enzymatic reaction is electrochemically detected. For this purpose a CdS\/ZnS quantum dot (QD) electrode is illuminated and the photocurrent response under fixed potential conditions is evaluated. The sequential enzyme reactions are first evaluated in solution. 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