{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:54:16Z","timestamp":1786978456916,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,13]],"date-time":"2019-01-13T00:00:00Z","timestamp":1547337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense","award":["HDTRA1-14-1-0064"],"award-info":[{"award-number":["HDTRA1-14-1-0064"]}]},{"name":"Defense Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defense","award":["HDTRA1-18-1-0014."],"award-info":[{"award-number":["HDTRA1-18-1-0014."]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Polymeric nanoparticles coated with membrane of intact red blood cells have emerged as biomimetic toxin nanosponges (RBC\u2010NS) that absorb and neutralize bacterial virulence factors associated with numerous bacterial infections. Despite its promise, a clear correlation between in vitro neutralization of complex bacterial toxins and in vivo therapeutic efficacy remains elusive. In this study, the whole secreted proteins (wSP) of methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) are collected to induce lethality in mice. The wSP preserve the complexity of bacterial virulence profile while avoiding the intricacy and dynamics of infections by live bacteria. RBC\u2010NS are first quantified for their neutralization capacity against the hemolytic activity of MRSA wSP in vitro. Using a mouse model, in vivo studies further demonstrate that, by neutralizing the hemolytic activity, RBC\u2010NS confer significant survival benefits against wSP\u2010induced lethality. Furthermore, when mice are challenged with a sublethal dosage of MRSA supernatant, RBC\u2010NS reduce lung damages and inhibit the activation of nuclear factor kappa B in the spleen. These results provide a systematic evaluation of RBC\u2010NS toward the treatment of severe MRSA infections such as MRSA bacteremia and MRSA\u2010induced sepsis.\n                  <\/jats:p>","DOI":"10.1002\/smll.201804994","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T02:50:02Z","timestamp":1547434202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Biomimetic Nanosponges Suppress In Vivo Lethality Induced by the Whole Secreted Proteins of Pathogenic Bacteria"],"prefix":"10.1002","volume":"15","author":[{"given":"Yijie","family":"Chen","sequence":"first","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengchun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronnie H.","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0637-0654","authenticated-orcid":false,"given":"Liangfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of NanoEngineering Chemical Engineering Program, and Moores Cancer Center University of California San Diego  La Jolla CA 92093 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