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While these polymersomes are highly stable in simulated intestinal fluids at extreme bile salt and osmolality conditions, they unexpectedly do not reduce plasmatic ammonia levels in cirrhotic rats after oral dosing. Incubation in dietary fiber hydrogels mimicking the colonic environment suggests that the vesicles are probably destabilized during the dehydration of the intestinal chyme. The findings question the relevance of commonly used simulated intestinal fluids for studying vesicular stability. With the encapsulation of a pH\u2010sensitive dye in the polymersome core, the local pH increase upon ammonia influx could be exploited to assess the ammonia concentration in the plasma of healthy and cirrhotic rats as well as in other fluids. Due to its high sensitivity and selectivity, this polymersome\u2010based assay could prove useful in the monitoring of hyperammonemic patients and in other applications such as drug screening tests.\n                  <\/jats:p>","DOI":"10.1002\/smll.201902347","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T04:57:02Z","timestamp":1573621022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["An Investigation of PS\u2010\n                    <i>b<\/i>\n                    \u2010PEO Polymersomes for the Oral Treatment and Diagnosis of Hyperammonemia"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1559-0950","authenticated-orcid":false,"given":"Simon","family":"Matoori","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Sciences Department of Chemistry and Applied Biosciences ETH Zurich  Zurich 8093 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