{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:33:15Z","timestamp":1793298795234,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T00:00:00Z","timestamp":1576454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31700831"],"award-info":[{"award-number":["31700831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002949","name":"Government of Jiangsu Province","doi-asserted-by":"publisher","award":["National Natural Science Foundation of China"],"award-info":[{"award-number":["National Natural Science Foundation of China"]}],"id":[{"id":"10.13039\/501100002949","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Reducing amyloid\u2010\u03b2 (A\u03b2) accumulation could be a potential therapeutic approach for Alzheimer's disease (AD). Particular functional biomolecules in exosomes vested by the microenvironment in which the original cells resided can be transferred to recipient cells to improve pathological conditions. However, there are few reports addressing whether exosomes derived from cells cultured on scaffolds with varying dimension can reduce A\u03b2 deposition or ameliorate cognitive decline for AD therapy. Herein, both 3D graphene scaffold and 2D graphene film are used as the matrix for human umbilical cord mesenchymal stem cell culture, from which the supernatants are obtained to isolate exosomes. The levels of 195 kinds of miRNAs and proteins, including neprilysin, insulin\u2010degrading enzyme and heat shock protein 70, in 3D\u2010cultured exosomes (3D\u2010Exo) are dramatically different from those obtained from 2D culture. Hence, 3D\u2010Exo could up\u2010regulate the expression of \u03b1\u2010secretase and down\u2010regulate the \u03b2\u2010secretase to reduce A\u03b2 production in both AD pathology cells and transgenic mice, through their special cargo. With rescuing A\u03b2 pathology, 3D\u2010Exo exerts enhanced therapeutic effects on ameliorating the memory and cognitive deficits in AD mice. These findings provide a novel clinical application for scaffold materials and functional exosomes derived from stem cells.<\/jats:p>","DOI":"10.1002\/smll.201906273","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T03:13:04Z","timestamp":1576465984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":141,"title":["The Regulatory Functionality of Exosomes Derived from hUMSCs in 3D Culture for Alzheimer's Disease Therapy"],"prefix":"10.1002","volume":"16","author":[{"given":"Lingyan","family":"Yang","sequence":"first","affiliation":[{"name":"School of Nano\u2010Tech and Nano\u2010Bionics University of Science and Technology of China  Hefei Anhui 230026 China"},{"name":"CAS Key Laboratory of Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou Jiangsu 215123 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