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During circulation and depending on the selected application (e.g., cancer drug delivery or immune modulators), nanoparticles are required to possess low or high interactions with cells in human blood and blood vessels to minimize side effects or maximize delivery efficiency. However, analysis of cellular interactions in blood vessels is challenging and is not yet realized due to the diverse components of human blood and hemodynamic flow in blood vessels. Here, the first comprehensive method to analyze cellular interactions of both synthetic and commercially available nanoparticles under human blood flow conditions in a microvascular network is developed. Importantly, this method allows to unravel the complex interplay of size, charge, and type of nanoparticles on their cellular associations under the dynamic flow of human blood. This method offers a unique platform to study complex interactions of any type of nanoparticles in human blood flow conditions and serves as a useful guideline for the rational design of liposomes and polymer nanoparticles for diverse applications in nanomedicine.<\/jats:p>","DOI":"10.1002\/smll.202002861","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T09:44:45Z","timestamp":1593078285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Cellular Interactions of Liposomes and PISA Nanoparticles during Human Blood Flow in a Microvascular Network"],"prefix":"10.1002","volume":"16","author":[{"given":"Mai N.","family":"Vu","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Convergent Bio\u2010Nano Science and Technology  Parkville VIC 3052 Australia"},{"name":"Monash Institute of Pharmaceutical Sciences Monash University  Parkville VIC 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