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However, targeted intracellular delivery of RNA therapeutics still remains a major challenge hindering the clinical translation. In this study, an elaborated plasmonic optoporation approach is proposed to efficiently and selectively transfect specific cells. The site\u2010specific optoporation is obtained by tuning the spectral range of a supercontinuum pulsed picosecond laser in order for each individual cell binding gold nanostar with their unique resonance peak to magnify the local field strength in the near\u2010infrared region and facilitate a selective delivery of small interfering RNA, messenger RNA, and Cas9\u2010ribonucleoprotein into human retinal pigment epithelial cells. Numerical simulations indicate that optoporation is not due to a plasma\u2010mediated process but rather due to a highly localized temperature rise both in time (few nanoseconds) and space (few nanometers). Taking advantage of the numerical simulation and fine\u2010tuning of the optical strategy, the perforated lipid bilayer of targeted cells undergoes a membrane recovery process, important to retain their viability. The results signify the prospects of antibody functionalized nanostar\u2010mediated optoporation as a simple and realistic gene delivery approach for future clinical practices.<\/jats:p>","DOI":"10.1002\/smll.202007577","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T07:50:11Z","timestamp":1617090611000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Antibody\u2010Functionalized Gold Nanostar\u2010Mediated On\u2010Resonance Picosecond Laser Optoporation for Targeted Delivery of RNA Therapeutics"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5202-8505","authenticated-orcid":false,"given":"Morteza","family":"Hasanzadeh Kafshgari","sequence":"first","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montreal  Montreal QC H3C3A7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7945-0121","authenticated-orcid":false,"given":"Leonidas","family":"Agiotis","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montreal  Montreal QC H3C3A7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9896-1341","authenticated-orcid":false,"given":"Isabelle","family":"Largilli\u00e8re","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montreal  Montreal QC H3C3A7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7175-481X","authenticated-orcid":false,"given":"Sergiy","family":"Patskovsky","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montreal  Montreal QC H3C3A7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2398-5602","authenticated-orcid":false,"given":"Michel","family":"Meunier","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montreal  Montreal QC H3C3A7 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"e_1_2_13_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3763"},{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00678"},{"key":"e_1_2_13_2_1","first-page":"6171","volume":"30","author":"Karra D.","year":"2010","journal-title":"J. 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