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Therefore, a resistance\u2010free antibacterial therapy is necessary to overcome MDR MRSA infections. In this study, an antibacterial nanorobot (Ab\u2010nanobot) is developed wherein a cell wall\u2010binding domain (CBD)\u2010endolysin, acting as a sensor, is covalently conjugated with an actuator consisting of an iron oxide\/silica core\u2013shell. The CBD\u2010endolysin sensor shows an excellent specificity to detect, bind, and accumulate on the <jats:italic>S. aureus<\/jats:italic> USA300 cell surface even in a bacterial consortium, and in host cell infections. Ab\u2010nanobot specifically captures and kills MRSA in response to medically approved radiofrequency (RF) electromagnetic stimulation (EMS) signal. When Ab\u2010nanobot receives the RF\u2010EMS signal on the cell surface, actuator induces cell death in MRSA with 99.999% removal within 20\u00a0min by cell\u2010wall damage via generation of localized heat and reactive oxygen species. The in vivo efficacy of Ab\u2010nanobot is proven using a mice subcutaneous skin infection model. Collectively, this study offers a nanomedical resistance\u2010free strategy to overcome MDR MRSA infections by providing a highly specific nanorobot for <jats:italic>S. aureus<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/smll.202100257","type":"journal-article","created":{"date-parts":[[2021,4,10]],"date-time":"2021-04-10T13:31:01Z","timestamp":1618061461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["An Antibacterial Nanorobotic Approach for the Specific Targeting and Removal of Multiple Drug\u2010Resistant <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"17","author":[{"given":"Nayab","family":"Batool","sequence":"first","affiliation":[{"name":"Department of Precision Medicine Institute for Antimicrobial Resistance Research and Therapeutics Graduate School of Basic Medical Sciences (GSBMS) Sungkyunkwan University School of Medicine  Suwon 16419 South Korea"},{"name":"Institute of Microbiology University of Agriculture  Faisalabad 38000 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seokyoung","family":"Yoon","sequence":"additional","affiliation":[{"name":"School of Advanced Materials Science and Engineering Sungkyunkwan University  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saba","family":"Imdad","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine Institute for Antimicrobial Resistance Research and Therapeutics Graduate School of Basic Medical Sciences (GSBMS) Sungkyunkwan University School of Medicine  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minsuk","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology Seoul National University of Science and Technology  Seoul 01811 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine Institute for Antimicrobial Resistance Research and Therapeutics Graduate School of Basic Medical Sciences (GSBMS) Sungkyunkwan University School of Medicine  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangryeol","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Food and Animal Biotechnology Department of Agricultural Biotechnology Research Institute of Agriculture and Life Sciences Seoul National University  Seoul 151\u2010921 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Heon","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Advanced Materials Science and Engineering Sungkyunkwan University  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhilesh Kumar","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine Institute for Antimicrobial Resistance Research and Therapeutics Graduate School of Basic Medical Sciences (GSBMS) Sungkyunkwan University School of Medicine  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2515-8894","authenticated-orcid":false,"given":"Kyeong Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine Institute for Antimicrobial Resistance Research and Therapeutics Graduate School of Basic Medical Sciences (GSBMS) Sungkyunkwan University School of Medicine  Suwon 16419 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,10]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00081-09"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.05.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.6.3854"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/598186"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00107"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15966-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/898457"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00194"},{"key":"e_1_2_7_9_1","first-page":"69","volume":"3","author":"Pai A.","year":"2013","journal-title":"Int. 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