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In this study, the first such model of the blood\u2010brain barrier (BBB\u2010on\u2010chip) featuring both isogenic human induced pluripotent stem cell (hiPSC)\u2010derived cells and continuous barrier integrity monitoring with &lt;2 min temporal resolution is reported. Its capabilities are showcased in the first microphysiological study of nitrosative stress and antioxidant prophylaxis. Relying on off\u2010stoichiometry thiol\u2013ene\u2013epoxy (OSTE+) for fabrication greatly facilitates assembly and sensor integration compared to the prevalent polydimethylsiloxane devices. The integrated cell\u2013substrate endothelial resistance monitoring allows for capturing the formation and breakdown of the BBB model, which consists of cocultured hiPSC\u2010derived endothelial\u2010like and astrocyte\u2010like cells. Clear cellular disruption is observed when exposing the BBB\u2010on\u2010chip to the nitrosative stressor linsidomine, and the barrier permeability and barrier\u2010protective effects of the antioxidant\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetylcysteine amide are reported. Using metabolomic network analysis reveals further drug\u2010induced changes consistent with prior literature regarding, e.g., cysteine and glutathione involvement. A model like this opens new possibilities for drug screening studies and personalized medicine, relying solely on isogenic human\u2010derived cells and providing high\u2010resolution temporal readouts that can help in pharmacodynamic studies.\n                  <\/jats:p>","DOI":"10.1002\/smll.202101785","type":"journal-article","created":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T12:56:43Z","timestamp":1624712203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Continuous Monitoring Reveals Protective Effects of\n                    <i>N<\/i>\n                    \u2010Acetylcysteine Amide on an Isogenic Microphysiological Model of the Neurovascular Unit"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4787-7785","authenticated-orcid":false,"given":"Isabelle","family":"Matthiesen","sequence":"first","affiliation":[{"name":"Division of Micro\u2010 and Nanosystems KTH Royal Institute of Technology  Malvinas V\u00e4g 10 pl 5 Stockholm 100 44 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-1702","authenticated-orcid":false,"given":"Dimitrios","family":"Voulgaris","sequence":"additional","affiliation":[{"name":"Division of Micro\u2010 and Nanosystems KTH Royal Institute of Technology  Malvinas V\u00e4g 10 pl 5 Stockholm 100 44 Sweden"},{"name":"AIMES, Center for Integrated Medical and Engineering Sciences Department of Neuroscience Karolinska Institute  Solnav\u00e4gen 9\/B8 Solna 171 65 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-9545","authenticated-orcid":false,"given":"Polyxeni","family":"Nikolakopoulou","sequence":"additional","affiliation":[{"name":"AIMES, Center for Integrated Medical and Engineering Sciences Department of Neuroscience Karolinska Institute  Solnav\u00e4gen 9\/B8 Solna 171 65 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2331-4833","authenticated-orcid":false,"given":"Thomas E.","family":"Winkler","sequence":"additional","affiliation":[{"name":"Division of Micro\u2010 and Nanosystems KTH Royal Institute of Technology  Malvinas V\u00e4g 10 pl 5 Stockholm 100 44 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5002-2537","authenticated-orcid":false,"given":"Anna","family":"Herland","sequence":"additional","affiliation":[{"name":"Division of Micro\u2010 and Nanosystems KTH Royal Institute of Technology  Malvinas V\u00e4g 10 pl 5 Stockholm 100 44 Sweden"},{"name":"AIMES, Center for Integrated Medical and Engineering Sciences Department of Neuroscience Karolinska Institute  Solnav\u00e4gen 9\/B8 Solna 171 65 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,26]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2020.103533"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cobme.2017.11.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201905055"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2019.04.006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snr.2021.100031"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4226"},{"key":"e_1_2_9_7_1","first-page":"5","volume":"2","author":"Kilic T.","year":"2018","journal-title":"Microphysiol. 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