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By screening biomaterials synthesized through a coacervation\u2010mediated self\u2010assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX\u20109 gene with high delivery efficiency. The self\u2010assembled injectable system is further utilized for 3D cell culture, segregated co\u2010culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self\u2010assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone\u2010shaped plate structures. Thus, the self\u2010assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.<\/jats:p>","DOI":"10.1002\/smll.202104758","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T08:55:23Z","timestamp":1644310523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Self\u2010Assembled Matrix System for Cell\u2010Bioengineering Applications in Different Dimensions, Scales, and Geometries"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9746-6004","authenticated-orcid":false,"given":"Yong","family":"Xu","sequence":"first","affiliation":[{"name":"B CUBE Center for Molecular Bioengineering Technische Universit\u00e4t Dresden  01307 Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle 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