{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:05:19Z","timestamp":1792649119159,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81925036"],"award-info":[{"award-number":["81925036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872814"],"award-info":[{"award-number":["81872814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parenteral vaccines typically can prime systemic humoral immune response, but with limited effects on cellular and mucosal immunity. Here, a subcutis\u2010to\u2010intestine cascade for navigating nanovaccines to address this limitation is proposed. This five\u2010step cascade includes lymph nodes targeting, uptaken by dendritic cells (DCs), cross\u2010presentation of antigens, increasing CCR9 expression on DCs, and driving CD103<jats:sup>+<\/jats:sup> DCs to mesenteric lymph nodes, in short, the LUCID cascade. Specifically, mesoporous silica nanoparticles are encapsulated with antigen and adjuvant toll\u2010like receptor 9 agonist cytosine\u2010phosphate\u2010guanine oligodeoxynucleotides, and further coated by a lipid bilayer containing all\u2010trans retinoic acid. The fabricated nanovaccines efficiently process the LUCID cascade to dramatically augment cellular and mucosal immune responses. Importantly, after being vaccinated with <jats:italic>Salmonella enterica serovar Typhimurium<\/jats:italic> antigen\u2010loaded nanovaccine, the mice generate protective immunity against challenge of <jats:italic>S. Typhimurium<\/jats:italic>. These findings reveal the efficacy of nanovaccines mediated subcutis\u2010to\u2010intestine cascade in simultaneously activating cellular and mucosal immune responses against mucosal infections.<\/jats:p>","DOI":"10.1002\/smll.202105530","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T11:36:46Z","timestamp":1637926606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Nanovaccines Mediated Subcutis\u2010to\u2010Intestine Cascade for Improved Protection against Intestinal Infections"],"prefix":"10.1002","volume":"18","author":[{"given":"Xiaofang","family":"Zhong","sequence":"first","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangsheng","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangsen","family":"Ou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairui","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yining","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyan","family":"Hao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuandong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Gong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhirong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0218-1664","authenticated-orcid":false,"given":"Xun","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education West China School of Pharmacy Sichuan University  Chengdu 610041 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.021"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc0927"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-017-0007-9"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00506-3"},{"key":"e_1_2_8_2_3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00975.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b00478"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201700426"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3254"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2019.02.035"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0261-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz4462"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12565"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00940-8"},{"key":"e_1_2_8_8_3","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12094"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0351-4"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2011.41"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.08.021"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201801067"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b04071"},{"key":"e_1_2_8_11_3","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00934"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44262"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.70"},{"key":"e_1_2_8_14_1","first-page":"1116","volume":"13","author":"Christensen D.","year":"2019","journal-title":"ACS Nano"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2019.04.005"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.08.007"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2008.07.010"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-09-245274"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02986"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni829"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-051116-052155"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.3"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aas9673"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10851"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.03.009"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.07.048"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01081.x"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18058-8"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202105530","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.202105530","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202105530","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T13:02:45Z","timestamp":1693227765000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202105530"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/smll.202105530"],"URL":"https:\/\/doi.org\/10.1002\/smll.202105530","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]},"assertion":[{"value":"2021-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2105530"}}