{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:53:23Z","timestamp":1772974403439,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T00:00:00Z","timestamp":1643414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32071407"],"award-info":[{"award-number":["32071407"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62003023"],"award-info":[{"award-number":["62003023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970760"],"award-info":[{"award-number":["31970760"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972842"],"award-info":[{"award-number":["81972842"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7212204"],"award-info":[{"award-number":["7212204"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0801702"],"award-info":[{"award-number":["2019YFA0801702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cell mechanical forces play fundamental roles in regulating cellular responses to environmental stimulations. The shortcomings of conventional methods, including force resolution and cellular throughput, make them less accessible to mechanical heterogeneity at the single\u2010cell level. Here, a DNA tensioner platform is introduced with high throughput (&gt;10 000 cells per chip) and pN\u2010level resolution. A microfluidic\u2010based cell array is trapped on \u201chairpin\u2010structured\u201d DNA tensioners that enable transformation of the mechanical information of living cells into fluorescence signals. By using the platform, one can identify enhanced mechanical forces of drug\u2010resistant cells as compared to their drug\u2010sensitive counterparts, and mechanical differences between metastatic tumor cells in pleural effusion and nonmetastatic histiocytes. Further genetic analysis traces two genes, VEGFA and MINK1, that may play deterministic roles in regulating mechanical heterogeneities. In view of the ubiquity of cells\u2019 mechanical forces in the extracellular microenvironment (ECM), this platform shows wide potential to establish links of cellular mechanical heterogeneity to genetic heterogeneity.<\/jats:p>","DOI":"10.1002\/smll.202106196","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T09:03:45Z","timestamp":1643447025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["High\u2010Throughput DNA Tensioner Platform for Interrogating Mechanical Heterogeneity of Single Living Cells"],"prefix":"10.1002","volume":"18","author":[{"given":"Xinxin","family":"Hang","sequence":"first","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqi","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaizai","family":"Dong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Li","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics, and China National Center for Bioinformation Chinese Academy of Sciences  No. 1 Beichen West Road, Chaoyang District Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Huang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics, and China National Center for Bioinformation Chinese Academy of Sciences  No. 1 Beichen West Road, Chaoyang District Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanruo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of RNA Biology CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences  No. 15 Datun Road, Chaoyang District Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research of Ministry of Education Department of Thoracic Surgery II Peking University Cancer Hospital &amp; Institute  No. 52 Fucheng Road, Haidian District Beijing 100142 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research of Ministry of Education Department of Thoracic Surgery II Peking University Cancer Hospital &amp; Institute  No. 52 Fucheng Road, Haidian District Beijing 100142 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianling","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing National Research Center for Information Science and Technology (BNRist) Institute of Microelectronics Tsinghua University  No. 30 Shuangqing Road, Haidian District Beijing 100084 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yubo","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jizhong","family":"Lou","sequence":"additional","affiliation":[{"name":"Key Laboratory of RNA Biology CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences  No. 15 Datun Road, Chaoyang District Beijing 100101 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiguo","family":"Yang","sequence":"additional","affiliation":[{"name":"Nebraska Center for Integrated Biomolecular Communication Department of Mechanical and Materials Engineering University of Nebraska\u2010Lincoln  Lincoln NE 68588 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2008-9631","authenticated-orcid":false,"given":"Lan","family":"Jiang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics, and China National Center for Bioinformation Chinese Academy of Sciences  No. 1 Beichen West Road, Chaoyang District Beijing 100101 China"},{"name":"College of Future Technology, and Sino\u2010Danish College University of Chinese Academy of Sciences  No. 19(A) Yuquan Road, Shijingshan District Beijing 100049 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6329-9850","authenticated-orcid":false,"given":"Lingqian","family":"Chang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering Beihang University  No. 37 Xueyuan Road, Haidian District Beijing 100083 China"},{"name":"School of Biomedical Engineering Research and Engineering Center of Biomedical Materials Anhui Medical University  No. 81 Meishan Road Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.08.041"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071813-105259"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2720"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9173-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b02376"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2010.89"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-016-0004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-018-0334-7"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2020.107648"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b03959"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7NR02940C"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/27\/6\/065102"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1218"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1821284116"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2007.388"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2008.100"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-0247"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/16219"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b07302"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600163113"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12939"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3564"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12814"},{"key":"e_1_2_9_24_1","first-page":"201609731","volume":"113","author":"Yang C.","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c07428"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b08359"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113086"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03935"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl501912g"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.1c00199"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt896"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2018.12.011"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.1992.10508670"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b09995"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/35036052"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201900043"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja2106477"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b11176"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3980"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2016.00267"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2017.98"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.1.127"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8079175"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03935"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aax3343"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-020-0298-8"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1348"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1613058113"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202007544"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5330-x"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.167"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25742"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07145-6"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b00716"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41567-020-0875-z"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010250"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b05286"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08428-2"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.10.033"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202106196","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.202106196","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202106196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T08:43:30Z","timestamp":1692434610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202106196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,29]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/smll.202106196"],"URL":"https:\/\/doi.org\/10.1002\/smll.202106196","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,29]]},"assertion":[{"value":"2021-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2106196"}}