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Furthermore, the platform to drive the differentiation of a certain DC subset without any additional biochemical manipulations has not yet been developed. Here, five types of polymer films with different hydrophobicity via an initiated chemical vapor deposition (iCVD) process to modulate the interactions related to cell\u2013substrate adhesion are introduced. Especially, poly(cyclohexyl methacrylate) (pCHMA) substantially enhances the expansion and differentiation of conventional type 1 DCs (cDC1s), the prime DC subset for antigen cross\u2010presentation, and CD8<jats:sup>+<\/jats:sup> T cell activation, by 4.8\u2010fold compared to the conventional protocol. The cDC1s generated from the pCHMA\u2010coated plates retain the bona fide DC functions including the expression of co\u2010stimulatory molecules, cytokine secretion, antigen uptake and processing, T cell activation, and induction of antitumor immune responses. To the authors\u2019 knowledge, this is the first report highlighting that the modulation of surface hydrophobicity of the culture plate can be an incisive approach to construct an advanced DC culture platform with high efficiency, which potentially facilitates basic research and the development of immunotherapy employing DCs.<\/jats:p>","DOI":"10.1002\/smll.202106648","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T09:31:09Z","timestamp":1647509469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Engineering of Surface Energy of Cell\u2010Culture Platform to Enhance the Growth and Differentiation of Dendritic Cells via Vapor\u2010Phase Synthesized Functional Polymer Films"],"prefix":"10.1002","volume":"18","author":[{"given":"Minseok","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering Korea Advanced Institute of 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