{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:37:46Z","timestamp":1785328666505,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"38","license":[{"start":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T00:00:00Z","timestamp":1660348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","award":["R01EB026207"],"award-info":[{"award-number":["R01EB026207"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A reactive oxygen species (ROS) responsive cleavable hierarchical metallic supra\u2010nanostructure (HMSN) is reported. HMSN structured with thin branches composed of primary gold (Au) nanocrystals and silver (Ag) nano\u2010linkers is synthesized by a one\u2010pot aqueous synthesis with a selected ratio of Au\/Ag\/cholate. ROS responsive degradability of HMSN is tested in the presence of endogenous and exogeneous ROS. Significant ROS\u2010responsive structural deformation of HMSN is observed in the ROS exposure with hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) solution. The ROS responsiveness of HMSN is significantly comparable with negligible structural changes of conventional spherical gold nanoparticles. The demonstrated ROS responsive degradation of HMSN is further confirmed in various in vitro ROS conditions of each cellular endogenous ROS and exogeneous ROS generated by photodynamic therapy (PDT) or X\u2010ray radiation. Then, in vivo ROS responsive degradability of HMSN is further evaluated with intratumoral injection of HMSN and exogeneous ROS generation via PDT in a mouse tumor model. Additional in vivo biodistribution and toxicity of intravenously administrated HMSN at 30\u2010day post\u2010injection are investigated for potential in vivo applications. The observed ROS responsive degradability of HMSN will provide a promising option for a type of ROS responsive\u2010multifunctional nanocarriers in cancer treatment and various biomedical applications.<\/jats:p>","DOI":"10.1002\/smll.202202694","type":"journal-article","created":{"date-parts":[[2022,8,13]],"date-time":"2022-08-13T13:45:35Z","timestamp":1660398335000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Reactive Oxygen Species Responsive Cleavable Hierarchical Metallic Supra\u2010Nanostructure"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5850-985X","authenticated-orcid":false,"given":"Hyunjun","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Radiology Feinberg School of Medicine Northwestern University  Chicago IL 60611 USA"},{"name":"Department of Biomedical Engineering University of Illinois at Chicago  Chicago IL 60607 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8754-9199","authenticated-orcid":false,"given":"Bongseo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Radiology Feinberg School of Medicine Northwestern University  Chicago IL 60611 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010Hyeok","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University  Seoburo 2066 Suwon Gyeonggi 16419 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha Eun","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University  Seoburo 2066 Suwon Gyeonggi 16419 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4614-0530","authenticated-orcid":false,"given":"Wooram","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology, College of Biotechnology and Bioengineering Sungkyunkwan University  Seoburo 2066 Suwon Gyeonggi 16419 Republic of Korea"},{"name":"Department of Biomedical Engineering McCormick School of Engineering  Evanston IL 60208 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6815-3319","authenticated-orcid":false,"given":"Dong\u2010Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Radiology Feinberg School of Medicine Northwestern University  Chicago IL 60611 USA"},{"name":"Department of Biomedical Engineering University of Illinois at Chicago  Chicago IL 60607 USA"},{"name":"Robert H. 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