{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:02:45Z","timestamp":1792465365225,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gene therapy using adeno\u2010associated virus (AAV) has potential as a radical treatment modality for genetic diseases such as sensorineural deafness. To establish clinical applications, it is necessary to avoid immune response to AAV by controlled release system of AAV. Here, a near\u2010infrared (NIR)\u2010triggered on\u2010demand AAV release system using alginate hydrogel microbeads with a heat transducer is proposed. By using a centrifuge\u2010based microdroplet shooting device, the microbeads encapsulating AAV with Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> microparticles (Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010MPs) as a heat transducer are fabricated. Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010MPs generated heat by NIR enhanced the diffusion speed of the AAV, resulting in the AAV being released from the microbeads. By irradiating the microbeads encapsulating fluorescent polystyrene nanoparticles (FP\u2010NPs) (viral model) with NIR, the fluorescence intensity decreased only for FP\u2010NPs with a diameter of 20\u00a0nm and not for 100 or 200\u00a0nm, confirming that this system can release virus with a diameter of several tens of nanometers. By irradiating NIR to the AAV\u2010encapsulating microbeads with Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010MPs, the AAV is released on demand, and gene transfection to cells by AAV is confirmed without loss of viral activity. The NIR\u2010triggered AAV release system proposed in this study increases the number of alternatives for the method of drug release in gene therapy.<\/jats:p>","DOI":"10.1002\/smll.202204139","type":"journal-article","created":{"date-parts":[[2022,12,10]],"date-time":"2022-12-10T02:01:28Z","timestamp":1670637688000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Near\u2010Infrared\u2010Triggered On\u2010Demand Controlled Release of Adeno\u2010Associated Virus from Alginate Hydrogel Microbeads with Heat Transducer for Gene Therapy"],"prefix":"10.1002","volume":"19","author":[{"given":"Shuhei","family":"Takatsuka","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Faculty of Science and Technology Keio University  3\u201014\u20101 Hiyoshi, Kohoku\u2010ku Yokohama 223\u20138522 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Faculty of Science and Technology Keio University  3\u201014\u20101 Hiyoshi, Kohoku\u2010ku Yokohama 223\u20138522 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Kurashina","sequence":"additional","affiliation":[{"name":"Division of Advanced Mechanical Systems Engineering Institute of Engineering Tokyo University of Agriculture and Technology  2\u201024\u201016 Hanamachi, Koganei\u2010shi Tokyo 184\u20138588 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0048-1580","authenticated-orcid":false,"given":"Hiroaki","family":"Onoe","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Faculty of Science and Technology Keio University  3\u201014\u20101 Hiyoshi, Kohoku\u2010ku Yokohama 223\u20138522 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan4672"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4025.949"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/357455a0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2019.01.019"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2141"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","unstructured":"N.Somia I. 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