{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:27:41Z","timestamp":1792160861382,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0112000"],"award-info":[{"award-number":["2019YFA0112000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFB1105600"],"award-info":[{"award-number":["2018YFB1105600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82102210"],"award-info":[{"award-number":["82102210"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue\u2010specific natural anisotropic microstructures play an important role in the normal functioning of tissues, yet they remain difficult to construct by current printing techniques. Herein, a stepwise algorithm\u2010assisted bioprinting technology for the construction of biomimetic tissues with a customizable anisotropic microstructure by combining the Adaptive Mesh Generation algorithm and the Greedy Search algorithm is developed. Based on the mechanical topology optimization design mechanism, the Adaptive Mesh Generation algorithm can generate controllable anisotropic mesh patterns with the minimum free energy in plane models according to tissue\u2010specific requirements. Subsequently, the Greedy Search algorithm can program the generated pattern data into optimized printing paths, effectively avoiding structural deformations caused by the multiple stacking of materials and reducing the printing time. The developed bioprinting technique is suitable for various types of bioinks including polymers, hydrogels, and organic\/inorganic complexes. After combining with a calcium phosphorus bioink, the compound algorithm\u2010assisted bioprinting technique successfully customizes femurs with biomimetic chemical compositions, anisotropic microstructures, and biological properties, demonstrating its effectiveness. Additionally, algorithm\u2010assisted bioprinting is generally suitable for most commercial extrusion bioprinters that function in the geometric code (G\u2010code) drive mode. Therefore, the algorithm\u2010assisted extrusion bioprinting technology offers an intelligent manufacturing strategy for the customization of anisotropic microstructures in biomimetic tissues.<\/jats:p>","DOI":"10.1002\/smll.202204316","type":"journal-article","created":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T01:37:06Z","timestamp":1664847426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Construction of Biomimetic Tissues with Anisotropic Structures via Stepwise Algorithm\u2010Assisted Bioprinting"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8201-1322","authenticated-orcid":false,"given":"Cuidi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialei","family":"Chen","sequence":"additional","affiliation":[{"name":"Georgia Tech Manufacturing Institute Georgia Institute of Technology  Atlanta GA 30332 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyang","family":"Jie","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopedic Implant Department of Orthopedic Surgery Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine  639 Zhizaoju Road Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kan","family":"Wang","sequence":"additional","affiliation":[{"name":"Georgia Tech Manufacturing Institute Georgia Institute of Technology  Atlanta GA 30332 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwu","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Orthopedic Implant Department of Orthopedic Surgery Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine  639 Zhizaoju Road Shanghai 200011 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianfu","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Wang","sequence":"additional","affiliation":[{"name":"Georgia Tech Manufacturing Institute Georgia Institute of Technology  Atlanta GA 30332 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-9582","authenticated-orcid":false,"given":"Wenguo","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  197 Ruijin 2nd Road Shanghai 200025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,3]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav9750"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav9051"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2017.10.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2498"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2014.09.009"},{"key":"e_1_2_7_6_1","first-page":"195","volume":"2","author":"Li C.","year":"2021","journal-title":"Eng. Regener."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13601"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.04.019"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aat4537"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14996-5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3413"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202104730"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c13905"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00126"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay1422"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.54864"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.50794"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.129570"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1908806116"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202108931"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201801076"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.01.057"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202108931"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1137\/S0036144503429121"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addma.2018.10.007"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addma.2016.06.006"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202103691"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202105883"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2021.05.018"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7NR08966J"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.08.059"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00238-2"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.07.018"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03350919"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(16)47003-8"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.130"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.12.028"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.45"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28673-2"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.05.003"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202204316","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.202204316","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202204316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T13:09:30Z","timestamp":1692623370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202204316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,3]]},"references-count":40,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1002\/smll.202204316"],"URL":"https:\/\/doi.org\/10.1002\/smll.202204316","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,3]]},"assertion":[{"value":"2022-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2204316"}}