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Here, the antibacterial activity and disease\u2010suppressive potential of biogenic copper nanoparticles (bio\u2010CuNPs) against bacterial fruit blotch (BFB), caused by <jats:italic>Acidovorax citrulli<\/jats:italic> (<jats:italic>Ac<\/jats:italic>), in watermelon (<jats:italic>Citrullus lanatus<\/jats:italic> L.) is discussed. CuNPs are extracellularly biosynthesized using a locally isolated bacterial strain\u00a0<jats:italic>Bacillus altitudinis<\/jats:italic> WM\u20102\/2, and have spherical shapes of 29.11\u201378.56\u00a0nm. Various metabolites, such as alcoholic compounds, carboxylic acids, alkenes, aromatic amines, and halo compounds, stabilize bio\u2010CuNPs. Foliar application of bio\u2010CuNPs increases the Cu accumulation in shoots\/roots (66%\/27%), and promotes the growth performance of watermelon plants by improving fresh\/dry weight (36%\/39%), through triggering various imperative physiological and biochemical processes. Importantly, bio\u2010CuNPs at 100 \u00b5g mL<jats:sup>\u22121<\/jats:sup> significantly suppress watermelon BFB through balancing reactive oxygen species system, improving photosynthesis capacity, and modulating stomatal immunity. Bio\u2010CuNPs show obvious antibacterial activity against <jats:italic>Ac<\/jats:italic> by inducing oxidative stress, biofilm inhibition, and cellular integrity disruption. These findings demonstrate that bio\u2010CuNPs can suppress watermelon BFB through direct antibacterial activity and induction of active immune response in watermelon plants, and highlight the value of this approach as a powerful tool to increase agricultural production and alleviate food insecurity.<\/jats:p>","DOI":"10.1002\/smll.202207136","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T02:03:28Z","timestamp":1672884208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["<i>Bacillus altitudinis<\/i>\u2010Stabilized Multifarious Copper Nanoparticles Prevent Bacterial Fruit Blotch in Watermelon (<i>Citrullus lanatus<\/i> L.): Direct Pathogen Inhibition, <i>In Planta<\/i> Particles Accumulation, and Host Stomatal Immunity Modulation"],"prefix":"10.1002","volume":"19","author":[{"given":"Muhammad","family":"Noman","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temoor","family":"Ahmed","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C.","family":"White","sequence":"additional","affiliation":[{"name":"The Connecticut Agricultural Experiment Station  New Haven CT 06504 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Mudassir","family":"Nazir","sequence":"additional","affiliation":[{"name":"Department of Agronomy College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Azizullah","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7192-5399","authenticated-orcid":false,"given":"Fengming","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c02323"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-080417-050108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/03235408.2021.1917952"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2022.20465431"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2020.128839"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-021-02579-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13593-015-0346-6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.8b03379"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-17-0221-R"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c00777"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9EN00265K"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-020-0110-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23084080"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010414"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20667"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants8010019"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5172"},{"key":"e_1_2_8_18_1","first-page":"661","volume":"2","author":"Apodaca S. 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