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It is noticed that regulating the gut microorganisms of bumblebees is of great significance for the maintenance of bumblebee health and disease treatment. Additionally, social bees are used as models to study regulatory control methods of gut bacteria in vivo. However, these methods lack precision and are not studied in bumblebees. In this study, nanotransducers are used for wireless spatiotemporal tuning of engineered bacteria in bumblebees. These nanotransducers are designed as 1D chains with smooth surfaces for easy transport in vivo, and temperature\u2010controlled engineered bacteria colonize the guts of microbial\u2010free bumblebees. Thermal production in the bumblebee gut is achieved using magnetothermal and photothermal methods in response to nanotransducers, resulting in significant target protein upregulation in engineered bacteria in the bumblebee gut. This advanced technology enables the precise control of engineered bacteria in the bumblebee gut. It also lays the foundation for the treatment of bumblebee intestinal parasitic diseases and the elimination of pesticide residues.<\/jats:p>","DOI":"10.1002\/smll.202301064","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T03:46:32Z","timestamp":1682999192000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Nanotransducer\u2010Enabled Wireless Spatiotemporal Tuning of Engineered Bacteria in Bumblebee"],"prefix":"10.1002","volume":"19","author":[{"given":"Xiaowen","family":"Cheng","sequence":"first","affiliation":[{"name":"College of Food Science and Nutritional Engineering China Agricultural University  Beijing 100083 China"},{"name":"Key Laboratory of Industrial Biocatalysis Ministry of Education Tsinghua University  Beijing 100084 China"},{"name":"Department of Chemical Engineering Tsinghua University  Beijing 100084 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