{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T01:32:18Z","timestamp":1775093538167,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"38","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJFS2145"],"award-info":[{"award-number":["JPMJFS2145"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJSP2129"],"award-info":[{"award-number":["JPMJSP2129"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20H01877"],"award-info":[{"award-number":["JP20H01877"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23KJ2081"],"award-info":[{"award-number":["23KJ2081"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H01188"],"award-info":[{"award-number":["22H01188"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K03560"],"award-info":[{"award-number":["22K03560"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JPMJFR213Y"],"award-info":[{"award-number":["JPMJFR213Y"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, a one\u2010step method is discussed for producing uniform cell\u2010sized microgels using glass capillaries filled with a binary polymer blend of polyethylene glycol (PEG) and gelatin. Upon decreasing temperature, phase separation of the PEG\/gelatin blends and gelation of gelatin occur, and then the polymer blend forms linearly aligned, uniformly sized gelatin microgels in the glass capillary. When DNA is added to the polymer solution, gelatin microgels entrapping DNA are spontaneously formed, and the DNA prevents the coalescence of the microdroplets even at temperatures above the melting point. This novel method to form uniform cell\u2010sized microgels may be applicable to other biopolymers. This method is expected to contribute to diverse materials science via biopolymer microgels and biophysics and synthetic biology through cellular models containing biopolymer gels.<\/jats:p>","DOI":"10.1002\/smll.202302193","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T22:21:41Z","timestamp":1684966901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Spontaneous Formation of Uniform Cell\u2010Sized Microgels through Water\/Water Phase Separation"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4369-372X","authenticated-orcid":false,"given":"Mayu","family":"Shono","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Materials Science Doshisha University  6100321 Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5215-5416","authenticated-orcid":false,"given":"Gen","family":"Honda","sequence":"additional","affiliation":[{"name":"Komaba Institute for Science Graduate School of Arts and Sciences The University of Tokyo  Komaba 3\u20108\u20101 Meguro Tokyo 153\u20108902 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7872-8286","authenticated-orcid":false,"given":"Miho","family":"Yanagisawa","sequence":"additional","affiliation":[{"name":"Komaba Institute for Science Graduate School of Arts and Sciences The University of Tokyo  Komaba 3\u20108\u20101 Meguro Tokyo 153\u20108902 Japan"},{"name":"Center for Complex Systems Biology Universal Biology Institute The University of Tokyo  Komaba 3\u20108\u20101 Meguro Tokyo 153\u20108902 Japan"},{"name":"Graduate School of Science The University of Tokyo  Hongo 7\u20103\u20101 Bunkyo Tokyo 113\u20100033 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2751-7136","authenticated-orcid":false,"given":"Kenichi","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Faculty of Life and Medical Sciences Doshisha University 6100394 Kyoto Japan"},{"name":"Center for Integrative Medicine and Physics Institute for Advanced Study Kyoto University  606 8501 Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1771-7660","authenticated-orcid":false,"given":"Akihisa","family":"Shioi","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science Doshisha University  6100321 Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioadv.2022.213156"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00339"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma5112292"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/life9010003"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201802288"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202200180"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00283"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2019.1579819"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.873854"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/la2016287"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575-016-0048-8"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-008-9238-8"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/b909597g"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CS00466A"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201903359"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SM01849J"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CC06606K"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000344"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201800066"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-020-00673-w"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2013.12.035"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416592111"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pol.1958.1203312618"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.83.061921"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN01209G"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c05857"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S331639"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00666"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00530"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00798"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-02990-w"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4767399"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2015.07.009"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0975"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.8b04304"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17774-5"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.07.020"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amc.2013.05.067"},{"key":"e_1_2_10_39_1","first-page":"1","author":"Benner P.","year":"2015","journal-title":"Science Open Research"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202201102"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2018.04.042"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202302193","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202302193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T13:15:37Z","timestamp":1695993337000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202302193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,24]]},"references-count":41,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1002\/smll.202302193"],"URL":"https:\/\/doi.org\/10.1002\/smll.202302193","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,24]]},"assertion":[{"value":"2023-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2302193"}}