{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:30:07Z","timestamp":1789025407338,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"43","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Accurately analyzing the functional activities of natural killer (NK) cells in clinical diagnosis remains challenging due to their coupling with other immune effectors. To address this, an integrated immune cell separator is required, which necessitates a streamlined sample preparation workflow including immunological cell isolation, removal of excess red blood cells (RBCs), and buffer exchange for downstream analysis. Here, a self\u2010powered integrated magneto\u2010microfluidic cell separation (SMS) chip is presented, which outputs high\u2010purity target immune cells by simply inputting whole blood. The SMS chip intensifies the magnetic field gradient using an iron sphere\u2010filled inlet reservoir for high\u2010performance immuno\u2010magnetic cell selection and separates target cells size\u2010selectively using a microfluidic lattice for RBC removal and buffer exchange. In addition, the chip incorporates self\u2010powered microfluidic pumping through a degassed polydimethylsiloxane chip, enabling the rapid isolation of NK cells at the place of blood collection within 40\u00a0min. This chip is used to isolate NK cells from whole blood samples of hepatocellular cancer patients and healthy volunteers and examined their functional activities to identify potential abnormalities in NK cell function. The SMS chip is simple to use, rapid to sort, and requires small blood volumes, thus facilitating the use of immune cell subtypes for cell\u2010based diagnosis.<\/jats:p>","DOI":"10.1002\/smll.202302809","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T07:53:33Z","timestamp":1687852413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Integrative Magneto\u2010Microfluidic Separation of Immune Cells Facilitates Clinical Functional Assays"],"prefix":"10.1002","volume":"19","author":[{"given":"Hee Sik","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Electrical Engineering Hanyang University  Seoul 04763 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeehun","family":"Park","sequence":"additional","affiliation":[{"name":"Soft Foundry Institute Seoul National University  1 Gwanak\u2010ro, Gwanak\u2010gu Seoul 08826 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