{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:25:15Z","timestamp":1791300315920,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFB3200801"],"award-info":[{"award-number":["2021YFB3200801"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC3300303"],"award-info":[{"award-number":["2021YFC3300303"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.62120106004"],"award-info":[{"award-number":["No.62120106004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urolithiasis stands as a prevalent ailment within the urinary system, with hyperoxaluria and hypocitraturia being the most frequent manifestations characterized by excessive oxalic acid (OA) and deficient citric acid (CA) levels in urine. Detecting these compounds in urine quantitatively holds paramount importance for early urolithiasis screening. Existing methodologies fall short in achieving simultaneous and on\u2010site identification of OA and CA, posing challenges for accurate urolithiasis screening. Addressing this concern, the study successfully accomplishes the concurrent identification of OA and CA in urine through a combination of dual\u2010spectral analysis and biomimetic peroxidase utilization. Bovine serum albumin and dithiothreitol\u2010modified copper nanoclusters (BSA\u2010DTT\u2010CuNCs) are employed as biomimetic peroxidases, effectively mitigating interference and enabling the simultaneous determination of OA and CA. The quantification range spans from 0 to 12\u00a0m<jats:sc>m<\/jats:sc> for OA and 0.5 to 2.5\u00a0m<jats:sc>m<\/jats:sc> for CA, with detection limits of 0.18 and 0.11\u00a0m<jats:sc>m<\/jats:sc>, respectively. To facilitate swift and on\u2010location urine analysis, a fully automated urine analyzer (FAUA) is introduced that streamlines the process of biomarker pretreatment and identification within urine samples. Validation with real urine samples from urolithiasis patients demonstrates the method's diagnostic precision, highlighting the dual\u2010spectral technique and analyzer's promising role in urolithiasis screening.<\/jats:p>","DOI":"10.1002\/smll.202304941","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:12:56Z","timestamp":1697094776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous Detection of Citric Acid and Oxalic Acid Based on Dual Spectrum and Biomimetic Peroxidase for Urolithiasis Screening with a Fully Automatic Urine Analyzer"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6978-6828","authenticated-orcid":false,"given":"Xianyou","family":"Sun","sequence":"first","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanchi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiyu","family":"Ma","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Sir Run Run Shaw Hospital Medical School of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shichao","family":"Tian","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liubing","family":"Kong","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoru","family":"Huang","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Liang","sequence":"additional","affiliation":[{"name":"Research Center for Quantum Sensing Zhejiang Lab  Hangzhou 310000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wan","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"},{"name":"State Key Laboratory of Transducer Technology Chinese Academy of Sciences  Shanghai 200050 China"},{"name":"Binjiang Institute of Zhejiang University  Hangzhou 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6474-2722","authenticated-orcid":false,"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University  Hangzhou 310027 China"},{"name":"State Key Laboratory of Transducer Technology Chinese Academy of Sciences  Shanghai 200050 China"},{"name":"Binjiang Institute of Zhejiang University  Hangzhou 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-017-2030-8"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4111\/icu.2017.58.5.299"},{"key":"e_1_2_9_3_1","volume":"12","author":"Romero V.","year":"2010","journal-title":"Rev Urol"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-020-01210-w"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.10.031"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2019.07.030"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-008-0960-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/end.2015.0676"},{"key":"e_1_2_9_10_1","first-page":"134","volume":"11","author":"Zuckerman J. M.","year":"2009","journal-title":"Rev Urol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0TB01108H"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112457"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2013.08.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201402137"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvs068"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CS00633H"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2017.12.020"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2014.05.123"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2019.02.010"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2017.12.009"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b07552"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c07431"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2020.124399"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AY01300K"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2017.05.038"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.121621"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.06.042"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-017-3904-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/am300408r"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-016-1915-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.11.056"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2017.10.021"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471143030.cba03hs52"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4183643"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.31857\/S0869-5652489144-48","volume":"489","author":"Ronzhin N. O.","year":"2019","journal-title":"\u0414\u043e\u043a\u043b\u0430\u0434\u044b \u0410\u043a\u0430\u0434\u0435\u043c\u0438\u0438 \u043d\u0430\u0443\u043a"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14943"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02867665"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202304941","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T14:30:20Z","timestamp":1709303420000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202304941"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,11]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/smll.202304941"],"URL":"https:\/\/doi.org\/10.1002\/smll.202304941","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,11]]},"assertion":[{"value":"2023-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2304941"}}