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The 2D GFe\u2013DAu\u2013D\/M tag with universal bacterial capture ability is fabricated through the layer\u2010by\u2010layer assembly of one layer of small Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    nanoparticles (NPs) and two layers of 30\u00a0nm AuNPs with a 0.5\u00a0nm built\u2010in nanogap on monolayer GO nanosheets followed by co\u2010modification with 4\u2010mercaptophenylboronic acid (MPBA) and 5,5\u2032\u2010dithiobis\u2010(2\u2010nitrobenzoic acid).The GFe\u2013DAu\u2013D\/M enabled the rapid enrichment of multiple bacteria by MPBA and quantitative analysis of target bacteria on test lines by specific antibodies, thus achieving multiple signal amplification of magnetic enrichment effect and multilayer dense hotspots and eliminating matrix interference in real\u2010world applications. The developed technology can directly and simultaneously diagnose three major pathogens (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , and\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    ) with detection limits down to the level of 10\u00a0cells\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The good performance of the proposed method in the detection of real urinary tract infection specimens is also demonstrated, suggesting the great potential of the GFe\u2013DAu\u2013D\/M\u2013ICA platform for the highly sensitive monitoring of bacterial infections or contamination.\n                  <\/jats:p>","DOI":"10.1002\/smll.202310014","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T04:29:53Z","timestamp":1704774593000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["2D Film\u2010Like Magnetic SERS Tag with Enhanced Capture and Detection Abilities for Immunochromatographic Diagnosis of Multiple Bacteria"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1396-6571","authenticated-orcid":false,"given":"Jiaxuan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine Guangdong Provincial 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