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As CTCs undergo epithelial\u2010mesenchymal transition (EMT), phenotype detection of heterogeneous CTCs based on EMT markers is of great significance. In this report, an integrated analytical strategy that can simultaneously capture and differentially detect epithelial\u2010 and mesenchymal\u2010expressed CTCs in bloods of non\u2010small cell lung cancer (NSCLS) patients is proposed. First, a commercial biomimetic polycarbonate (PCTE) microfiltration membrane is employed as the capture interface for heterogenous CTCs. Meanwhile, differential detection of the captured CTCs is realized by preparing two distinct CdTe quantum dots (QDs) with red and green emissions, attached with EpCAM and Vimentin aptamers, respectively. For combined analysis, a polydimethylsiloxane (PDMS) chip with simple structure is designed, which integrates the membrane capture and QDs\u2010based phenotype detection of CTCs. This chip not only implements the analysis of the number of CTCs down to 2 cells\u00a0mL<jats:sup>\u22121<\/jats:sup>, but enables EMT process tracking according to the specific signals of the two QDs. Finally, this method is successfully applied to inspect the correlations of numbers or proportions of heterogenous CTCs in 94 NSCLS patients with disease stage and whether there is distant metastasis.<\/jats:p>","DOI":"10.1002\/smll.202310360","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T05:01:12Z","timestamp":1714712472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Circulating Tumor Cell Phenotype Detection and Epithelial\u2010Mesenchymal Transition Tracking Based on Dual Biomarker Co\u2010Recognition in an Integrated PDMS Chip"],"prefix":"10.1002","author":[{"given":"Hao","family":"Xu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy Xuzhou Medical University  209 Tongshan Road Xuzhou 221004 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