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This study introduces a Lab\u2010in\u2010a\u2010Vial (LV) rapid diagnostic platform, featuring hydrangea\u2010like platinum nanozymes (PtNH), for rapid, accurate detection and quantification of protein biomarkers on\u2010site within 15\u00a0min. This method significantly enhances detection sensitivity for various biomarkers in body fluids, surpassing traditional methods such as enzyme\u2010linked immunosorbent assays (ELISA) and lateral flow assays (LFA) by \u2248250 to 1300 times. The LV platform uses a glass vial coated with specific bioreceptors such as antigens or antibodies, enabling rapid in vitro evaluation of disease risk from small fluid samples, similar to a personal ELISA\u2010like point\u2010of\u2010care test (POCT). It overcomes challenges in on\u2010site biomarker detection, allowing both detection and quantification through a portable wireless spectrometer for healthcare internet of things (H\u2010IoT). The platform's effectiveness and adaptability are confirmed using IgG\/IgM antibodies from SARS\u2010CoV\u20102 infected patients and nuclear matrix protein (NMP22) from urothelial carcinoma (UC) patients as biomarkers. These tests demonstrated its accuracy and flexibility. This approach offers vast potential for diverse disease applications, provided that the relevant protein biomarkers in bodily fluids are identified.<\/jats:p>","DOI":"10.1002\/smll.202400878","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T10:00:50Z","timestamp":1722938450000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lab\u2010in\u2010a\u2010Vial Rapid Test for Internet of Things\u2010Embedded Point\u2010of\u2010Healthcare Protein Biomarker Detection in Bodily Fluids"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6991-6223","authenticated-orcid":false,"given":"Nan\u2010Si","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering National Cheng Kung University  Tainan 70101 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3008-5882","authenticated-orcid":false,"given":"Ying\u2010Pei","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Materials and Optoelectronic Science National Sun Yat\u2010sen University  Kaohsiung 80424 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-9649","authenticated-orcid":false,"given":"Hao\u2010Han","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering National Cheng Kung University  Tainan 70101 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng\u2010Fan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology Kaohsiung Medical University  Kaohsiung 80708 Taiwan"},{"name":"Center for Tropical Medicine and Infectious Diseases Research Kaohsiung Medical University  Kaohsiung 80708 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"See\u2010Tong","family":"Pang","sequence":"additional","affiliation":[{"name":"Division of Urology Department of Surgery Chang Gung Memorial Hospital Linkou  Taoyuan 33305 Taiwan"},{"name":"Department of Medicine College of Medicine Chang Gung University  Taoyuan 33302 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih\u2010Yen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology Kaohsiung Medical University  Kaohsiung 80708 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rung\u2010Ywan","family":"Tsai","sequence":"additional","affiliation":[{"name":"Caduceus Biotechnology Inc.  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