{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:20:49Z","timestamp":1769505649777,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010713","name":"Indian Institute of Science Education and Research Bhopal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008790","name":"National Institute of Technology Rourkela","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008790","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aberrant accumulation of cytotoxic protein aggregates is a hallmark of various neurodegenerative and non\u2010neurodegenerative ailments, necessitating the development of sensitive and selective tools for their detection. Herein, we report a series of morpholine\u2010anchored fluorescent probes, denoted as <jats:bold>SC\u2010nmor<\/jats:bold> (<jats:italic>n<\/jats:italic> = 2, 4, 6), designed for facile visualization of protein aggregates. These probes display notable changes in their photophysical properties upon binding with protein aggregates, owing to their high sensitivity to the fibrillar microenvironment. Specifically, the <jats:bold>SC\u20104mor<\/jats:bold> probe demonstrates strong selectivity for aggregated insulin proteins over native insulin, accompanied by a significant enhancement in fluorescence lifetime. Live\u2010cell imaging reveals an exclusive localization at the lysosomal compartment. This feature enables the visualization of lysosomal accumulated protein fibrils induced with pepstatin A. Additionally, in vivo assessments on genetically mutated and dietary\u2010modified <jats:italic>Drosophila<\/jats:italic> <jats:italic>melanogaster<\/jats:italic>, representing neurodegenerative and non\u2010neurodegenerative disease models, demonstrate staining of protein aggregates. The enhanced emission from the eye lobes of A<jats:italic>\u03b2<\/jats:italic>\u2010mutated and HSD brain samples, suggesting that <jats:bold>SC\u20104mor<\/jats:bold> can exhibit adequate retention in the brain with minimal biological toxicity. <jats:bold>SC\u20104mor<\/jats:bold> also shows its capability to cross the blood\u2013brain barrier in mice model. Consequently, <jats:bold>SC\u20104mor<\/jats:bold> emerges as a promising marker for detecting and monitoring neurotoxic protein fibrillation in live cells and animal models, offering potential insights into the pathogenesis and progression of protein aggregation.<\/jats:p>","DOI":"10.1002\/smll.202404008","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T07:21:20Z","timestamp":1734506480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Morpholine Anchored Fluorogenic Toolkit: Unveiled Disease Allied Protein Fibrillation in Lysosomal Compartment of Live\u2010Cell and <i>Drosophila<\/i> Models"],"prefix":"10.1002","volume":"21","author":[{"given":"Kavyashree","family":"P.","sequence":"first","affiliation":[{"name":"Bionanotechnology Laboratory Department of Chemistry Indian Institute of Science Education and Research Bhopal  Bhopal Bypass Road, Bhauri Bhopal Madhya Pradesh 462066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Wilson","sequence":"additional","affiliation":[{"name":"Bionanotechnology Laboratory Department of Chemistry Indian Institute of Science Education and Research Bhopal  Bhopal Bypass Road, Bhauri Bhopal Madhya Pradesh 462066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akshay","family":"Silswal","sequence":"additional","affiliation":[{"name":"Bionanotechnology Laboratory Department of Chemistry Indian Institute of Science Education and Research Bhopal  Bhopal Bypass Road, Bhauri Bhopal Madhya Pradesh 462066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lopamudra","family":"Mishra","sequence":"additional","affiliation":[{"name":"Neural Developmental Biology Laboratory Department of Life Science National Institute of Technology (NIT)  Rourkela Odisha 769008 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debapriya","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Indian Institute of Science Education and Research Bhopal  Bhopal Bypass Road, Bhauri Bhopal Madhya Pradesh 462066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monalisa","family":"Mishra","sequence":"additional","affiliation":[{"name":"Neural Developmental Biology Laboratory Department of Life Science National Institute of Technology (NIT)  Rourkela Odisha 769008 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8891-416X","authenticated-orcid":false,"given":"Apurba Lal","family":"Koner","sequence":"additional","affiliation":[{"name":"Bionanotechnology Laboratory Department of Chemistry Indian Institute of Science Education and Research Bhopal  Bhopal Bypass Road, Bhauri Bhopal Madhya Pradesh 462066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a016840"},{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201607005"},{"key":"e_1_2_9_1_3","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051131"},{"key":"e_1_2_9_1_4","volume":"42","author":"He Y.","year":"2024","journal-title":"Med. Res. Rev."},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2016.08.014"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12056"},{"key":"e_1_2_9_2_3","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2745"},{"key":"e_1_2_9_2_4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24900-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21394"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-022-01084-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12631"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10010138"},{"key":"e_1_2_9_4_3","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-020-00649-8"},{"key":"e_1_2_9_4_4","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105919"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.10.3861"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.10998"},{"key":"e_1_2_9_5_3","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90324-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2020.00037"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1NJ01606G"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2286"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-017-9769-4"},{"key":"e_1_2_9_8_3","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-061516-044518"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/416507a"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/C5SC00754B"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01046"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c01281"},{"key":"e_1_2_9_10_3","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202311670"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00647-2"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c05466"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2SC05004H"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.5b00324"},{"key":"e_1_2_9_12_3","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c00587"},{"key":"e_1_2_9_12_4","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC04730A"},{"key":"e_1_2_9_12_5","doi-asserted-by":"publisher","DOI":"10.1039\/C9CC09233A"},{"key":"e_1_2_9_12_6","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00379"},{"key":"e_1_2_9_12_7","doi-asserted-by":"publisher","DOI":"10.1039\/D3TB02346J"},{"key":"e_1_2_9_12_8","doi-asserted-by":"publisher","DOI":"10.1039\/D4TB00572D"},{"key":"e_1_2_9_12_9","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c04721"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2TB00879C"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.2c00361"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.1c00810"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi002555c"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-122313-085916"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2011.0370"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800699v"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.2.F23"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.169101"},{"key":"e_1_2_9_18_3","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.023762"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00051"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.728407"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14020235"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0253-x"},{"key":"e_1_2_9_22_1","volume":"3","author":"Haque M. K.","year":"2017","journal-title":"Adv. Biotech & Micro."},{"key":"e_1_2_9_23_1","first-page":"Ch. 3","volume-title":"Preclinical Animal Modeling in Medicine","author":"Zoha S.","year":"2021"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2020.109739"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2015.04.005"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13204-023-02988-y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp310232b"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.6b10078"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2016.01.005"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-022-01104-3"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202404008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T05:23:25Z","timestamp":1749187405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202404008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,17]]},"references-count":61,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/smll.202404008"],"URL":"https:\/\/doi.org\/10.1002\/smll.202404008","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,17]]},"assertion":[{"value":"2024-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2404008"}}