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Here, a magnetically assisted dual\u2010signal output LFA platform is developed for the ultrasensitive, universal, and flexible detection of viruses. A \u201cthree\u2010in\u2010one\u201d multifunctional probe (MAuDQD) is prepared using a 180\u00a0nm Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    core to load numerous Au nanoparticles (NPs) and two layers of QDs, which can substantially improve the sensitivity of LFA through coupling with the effects of magnetic enrichment and colorimetric\/fluorescent enhancement. Wheat germ agglutinin (WGA)\u2010modified MAuDQD attained the broad\u2010spectrum capture viral membrane proteins and the colorimetric\/fluorescent dual\u2010mode detection of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) and monkeypox virus (MPXV) on the LFA strip. In the colorimetric mode, the target viruses detected directly, with the visual sensitivity reaching 0.1\u20130.5\u00a0ng\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    and the fluorescent mode supported quantitative analysis of SARS\u2010CoV\u20102\/MPXV with limits of detection decreasing to pg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    level. Practicability of the MAuDQD@WGA\u2010LFA is verified through the detection of 33 real clinical samples, showing the proposed assay has a great potential to become a sensitive, accurate, and universal tool for on\u2010site monitoring of viral infections.\n                  <\/jats:p>","DOI":"10.1002\/smll.202406053","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T01:41:46Z","timestamp":1729647706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Wheat Germ Agglutinin\u2010Modified \u201cThree\u2010in\u2010One\u201d Multifunctional Probe Driven Broad\u2010Spectrum and Flexible Immunochromatographic Diagnosis of viruses With High Sensitivity"],"prefix":"10.1002","volume":"21","author":[{"given":"Ting","family":"Wu","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology College of Chemistry and Life Science Beijing University of 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