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In this study, a rationally designed multifunctional metallic nano\u2010antibiotic (MNAB) system using an in\u2010situ reduction method is developed. The strategic incorporation of cuprous species substantially enhanced antimicrobial activity against clinically isolated strains, including two Gram\u2010positive and four Gram\u2010negative bacterial species. Remarkably, the system exhibited minimum inhibitory concentrations (MIC) of \u224820\u00a0ppm for Gram\u2010positive bacteria and \u224860\u00a0ppm for Gram\u2010negative bacteria, demonstrating efficacy on par with conventional antibiotics. Mechanistic investigations revealed that MNABs exert their antimicrobial effects through potent oxidative damage to cellular membranes while simultaneously modulating multiple gene clusters, confirming their broad\u2010spectrum antimicrobial capabilities. 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