{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:42:54Z","timestamp":1787316174354,"version":"build-2736575974"},"reference-count":89,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:00:00Z","timestamp":1739059200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009252","name":"Science for Life Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009252","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["101002582"],"award-info":[{"award-number":["101002582"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Superparamagnetic iron oxide nanoparticles (SPIONs) exhibit unique properties for diverse biomedical applications, including drug delivery and diagnostic imaging. Actively targeted SPIONs enhance delivery to diseased sites, reducing side effects and enhancing treatment efficacy. However, the development of reproducible functionalization protocols is challenged by the erratic behavior of nanoparticles in suspensions, such as agglomeration and sedimentation. In this study, a functionalization method is developed and systematically optimized to attach the Fc\u2010region of antibodies onto silica\u2010coated SPIONs via click chemistry, ensuring controlled ligand orientation on the particle surface. The synthesis and successive modifications of silica\u2010coated SPIONs with organic moieties are presented resulting in the final click conjugation with antibodies targeting intercellular adhesion molecule 1 (ICAM1). This protein is upregulated on epithelial cell surfaces during gastrointestinal inflammation. Thermogravimetric analysis and infrared spectroscopy confirm successful SPION functionalization after each modification step. Cell viability assessment indicates no adverse effects of bioconjugated particles. Quantitative elemental analysis reveals significantly higher iron concentration in inflammation\u2010induced Caco\u20102 cells exposed to ICAM1\u2010modified particles compared to non\u2010conjugated counterparts. Furthermore, laser scanning confocal microscopy of these cells suggests surface interaction and internalization of bioconjugated SPIONs, underscoring their potential for targeted imaging and therapy in inflammatory diseases.<\/jats:p>","DOI":"10.1002\/smll.202407883","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:17:26Z","timestamp":1739251046000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Click Chemistry\u2010Based Bioconjugation of Iron Oxide Nanoparticles"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0589-546X","authenticated-orcid":false,"given":"Shno","family":"Asad","sequence":"first","affiliation":[{"name":"Department of Pharmacy Science for Life Laboratory Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4364-8571","authenticated-orcid":false,"given":"David","family":"Ahl","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology Science for Life Laboratory Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9405-8278","authenticated-orcid":false,"given":"Yael del Carmen","family":"Su\u00e1rez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Science for Life Laboratory Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0359-5970","authenticated-orcid":false,"given":"M\u00e1t\u00e9","family":"Erd\u00e9lyi","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u2013 BMC Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2387-0266","authenticated-orcid":false,"given":"Mia","family":"Phillipson","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology Science for Life Laboratory Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6514-8960","authenticated-orcid":false,"given":"Alexandra","family":"Teleki","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Science for Life Laboratory Uppsala University  Uppsala SE\u201075123 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-020-0090-8"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S153758"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b06040"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2015.04.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.12.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2217\/17435889.2.2.153"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsta.2009.0273"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b04873"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-021-00561-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjnano.14.75"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700306"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2022-065186"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgast-2022-000958"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/439389"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0171-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201600550"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.9b02276"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40580-021-00274-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200800714"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200701308"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.200500360"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/23\/48\/485103"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201000279"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201301417"},{"key":"e_1_2_8_25_1","first-page":"2595","volume":"10","author":"Ndong C.","year":"2015","journal-title":"Int J Nanomedicine"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6RA06076E"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.6b01142"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2009.00021"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NR04683E"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CC01848A"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201303629"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc400102w"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2020.115808"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.5b00224"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201402606"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.025"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2016.03.022"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn505857g"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-820757-4.00003-X"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201906539"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1620145114"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmmi.1473"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2014.05.003"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2020.11.001"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm803153m"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2509(03)00022-8"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/b711652g"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200602866"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6RA03115C"},{"key":"e_1_2_8_50_1","first-page":"23","volume":"8","author":"Ansari S. R.","year":"2024","journal-title":"ACS Nano"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201303416"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600725"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c07158"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2014.03.130"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2020.100931"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym11030537"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-84770-0"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/la8024827"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2013.11.017"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios13010036"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/la403269u"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2015.01.107"},{"key":"e_1_2_8_63_1","unstructured":"E. C. Agency \u201cCandidate List of substances of very high concern for Authorisation \u201d can be found underhttps:\/\/echa.europa.eu\/candidate\u2010list\u2010table."},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr800323s"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03135-9"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9PY01136F"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.36.5.724"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.106"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202402502"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.medidd.2021.100110"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo011148j"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics10020057"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c03556"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.impact.2019.100165"},{"key":"e_1_2_8_76_1","first-page":"2","volume":"14","author":"Le Khanh H. P.","year":"2022","journal-title":"Polymers (Basel)"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7RA00861A"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppsc.201300357"},{"key":"e_1_2_8_79_1","volume-title":"Biological Evaluation of Medical Devices \u2013 Part Vol. 5: Tests for in Vitro Cytotoxicity","author":"I. O. Standardization","year":"2009"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2010.04.002"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.303"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.5b01326"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp300649z"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.020"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201000528"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00858-8"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.06.007"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1926.10502161"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1027"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202407883","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/smll.202407883","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202407883","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T13:45:40Z","timestamp":1779025540000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202407883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,9]]},"references-count":89,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/smll.202407883"],"URL":"https:\/\/doi.org\/10.1002\/smll.202407883","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,9]]},"assertion":[{"value":"2024-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2407883"}}