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The barcodes, characterized by core\u2010shell structures, are fabricated through partial replication of periodically ordered hexagonally close\u2010packed silicon dioxide beads. Consequently, the opal hydrogel shell of these barcodes features abundant interconnected pores that provide a substantial surface area for probe immobilization. Furthermore, the inherent structural colors remain stable during detection events due to the robust mechanical strength of the barcode cores. This integration of CRISPR\/Cas13a and HCR leverages both the highly specific RNA recognition capabilities and trans\u2010cleavage activity of Cas13a while employing HCR to enhance sensitivity. Upon encountering target RNA, Cas13a cleaves a hairpin probe, thereby initiating subsequent HCR amplification for enhanced detection sensitivity. Our method demonstrates high accuracy and sensitivity in multiplexed detection of SARS\u2010CoV\u20102, Flu A and Flu B RNA with a limit\u2010of\u2010detection as low as 200 aM. Importantly, this assay also exhibits acceptable accuracy in repeated clinical sample testing. Thus, our platform represents a promising strategy for highly sensitive multiplexed virus detection in clinical.<\/jats:p>","DOI":"10.1002\/smll.202408725","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T02:12:56Z","timestamp":1737511976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CRISPR\/Cas13a\u2010Enhanced Porous Hydrogel Encapsulated Photonic Barcodes for Multiplexed Detection of Virus"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7192-6340","authenticated-orcid":false,"given":"Dagan","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Translational Medicine The Affiliated Drum Tower Hospital of Nanjing University Medical School  Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine The Affiliated Drum Tower Hospital of Nanjing University Medical School  Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine The Affiliated Drum Tower Hospital of Nanjing University Medical School  Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qichen","family":"Luan","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine The Affiliated Drum Tower Hospital of Nanjing University Medical School  Nanjing 210008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj1281"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abi8452"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe3703"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00939-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn3481"},{"key":"e_1_2_9_7_1","volume":"6","author":"Zhou L.","year":"2020","journal-title":"Sci. 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