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A cell model of HSN\u20101A is developed by overexpressing wild\u2010type and mutant SPTLC1 genes (C133W, C133Y, V144D). Cells are cultured on plastic and gold substrates, with brief electrical stimulation applied to the gold\u2010grown cells. Atomic force microscopy (AFM) is used to measure Young's modulus, indentation, and energy dissipation. Finite Element Method and non\u2010linear modeling validate the results. In the absence of stimulation, mutant cells show lower stiffness compared to non\u2010transfected cells, indicating a direct biomechanical impact of the mutations. Brief electrical stimulation significantly increases the stiffness of mutant cells, particularly in C133W (99%), C133Y (100%), and V144D (111%) variants, despite the mutations. Energy dissipation of stimulated V144D cells decreases to levels comparable to untreated non\u2010transfected cells. The simulations support the AFM measurements, demonstrating that brief electrical stimulation can partially reverse the biomechanical effects of gene mutations.<\/jats:p>","DOI":"10.1002\/smll.202408941","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T01:08:56Z","timestamp":1738631336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Brief Electrical Stimulation on Cell Biomechanics in Hereditary Sensory Neuropathy"],"prefix":"10.1002","volume":"21","author":[{"given":"Daniel","family":"Ta","sequence":"first","affiliation":[{"name":"School of Science Western Sydney University  Penrith New South Wales 2751 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anu","family":"Anthony","sequence":"additional","affiliation":[{"name":"School of Science Western Sydney University  Penrith New South Wales 2751 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