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Fibrosis is a key aspect of endometriosis, yet current cell models to study it remain limited. This work aims to bridge the translational gap between in vitro fibrosis research and preclinical testing of non\u2010hormonal drug candidates. When grown in a 3D matrix of sustainably produced silk protein functionalized with a fibronectin\u2010derived cell adhesion motif (FN\u2010silk), endometrial stromal and epithelial cells respond to transforming growth factor beta\u20101 (TGF\u2010\u03b21) in a physiological manner as probed at the messenger RNA (mRNA) level. For stromal cells, this response to TGF\u2010\u03b21 is not observed in spheroids, while epithelial cell spheroids behave similarly to epithelial cell FN\u2010silk networks. 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Overall, endometrial stromal cells cultured in FN\u2010silk networks\u2014which are composed of a sustainably produced, fully defined FN\u2010silk protein\u2014recapitulate fibrotic cellular behavior with high fidelity and enable antifibrotic drug testing.<\/jats:p>","DOI":"10.1002\/smll.202409126","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T03:27:51Z","timestamp":1739935671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Fibronectin (FN)\u2010Silk\u00a03D Cell Culture Model as a Screening Tool for Repurposed Antifibrotic Drug Candidates for\u00a0Endometriosis"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5074-1614","authenticated-orcid":false,"given":"Sarah","family":"Teworte","sequence":"first","affiliation":[{"name":"Pharmaceutical Technology Research Group Department of Chemistry, Biochemistry and Pharmaceutical Sciences 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