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Current therapies primarily inhibit vascular endothelial growth factor (VEGF) signaling; however, targeting additional pathways may improve outcomes. A bispecific peptide (BsPt) is developed that targets VEGFR2 and integrin \u03b1v\u03b23 to treat retinal neovascularization (RNV) and choroidal neovascularization (CNV) more effectively. BsPt binds to both receptors on endothelial cells (ECs) and forms nanofibrous networks on their surfaces, resulting in potent NV inhibition. In vitro, BsPt suppressed retinal EC migration and tube formation. Intravitreal BsPt injection outperformed aflibercept in mouse models of oxygen\u2010induced retinopathy (OIR) and laser\u2010induced CNV. RNA sequencing revealed that BsPt\u2010induced nanofibers upregulated extracellular matrix (ECM) and cell adhesion genes, which may contribute to anti\u2010angiogenesis and anti\u2010leakage effects. 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