{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:27:38Z","timestamp":1789144058238,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T00:00:00Z","timestamp":1734912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003382","name":"Core Research for Evolutional Science and Technology","doi-asserted-by":"publisher","award":["JPMJCR23L2"],"award-info":[{"award-number":["JPMJCR23L2"]}],"id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24KJ1129"],"award-info":[{"award-number":["24KJ1129"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H04409"],"award-info":[{"award-number":["23H04409"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K05108"],"award-info":[{"award-number":["21K05108"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper discusses the controlled morphology of hierarchical liquid crystalline DNA assemblies. Through a process of heating and slow cooling, double\u2010stranded DNAs (dsDNAs) having 23 complementary bases and two base overhangs (a pair of 25mer oligonucleotides) spontaneously assemble into micro\u2010sized hexagonal platelets in a solution containing poly(ethylene glycol) (PEG) and salt. Remarkably, the addition of a shorter dsDNA with AA\/TT overhangs (a pair of 18mer oligonucleotides) to a PEG\u2010salt solution of 25mer DNA with AA\/TT overhangs results in the formation of molecular tubes, each with a central blockage. In contrast, the addition of 18mer DNA with GG\/CC overhangs leads to the formation of hexagonal frames from hexagonal platelets. In the assemblies, 18mer DNA is more predominant at the edges, while the central nuclei are composed of 25mer DNA. X\u2010ray scattering measurements reveal that both molecular tubes and hexagonal frames form hexagonal columnar liquid crystalline assemblies. These self\u2010assemblies are thought to result from depletion attraction due to PEG and end\u2010to\u2010end stacking between dsDNA overhangs. Differences in the melting temperatures between longer and shorter dsDNAs enable the stepwise formation of hierarchical assemblies, while variations in overhang sequences modulate the overall morphology by altering the growth direction of the assemblies.<\/jats:p>","DOI":"10.1002\/smll.202410243","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T09:32:35Z","timestamp":1734946355000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Controlling the Hierarchical Assembly of DNA\u2010Based Hexagonal Microstructures"],"prefix":"10.1002","volume":"21","author":[{"given":"Tetsunao","family":"Makino","sequence":"first","affiliation":[{"name":"Department of Engineering Science Graduate School of Informatics and Engineering The University of Electro\u2010Communications  1\u20105\u20101 Chofugaoka Chofu Tokyo 182\u20138585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9814-654X","authenticated-orcid":false,"given":"Takashi","family":"Kajitani","sequence":"additional","affiliation":[{"name":"Core Facility Center Research Infrastructure Management Center Institute of Science Tokyo  4259 Nagatsuta, Midori\u2010ku Yokohama 226\u20138503 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5819-802X","authenticated-orcid":false,"given":"Makiko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Engineering Science Graduate School of Informatics and Engineering The University of Electro\u2010Communications  1\u20105\u20101 Chofugaoka Chofu Tokyo 182\u20138585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,23]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2745"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.3c00028"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06597"},{"key":"e_1_2_8_4_1","first-page":"1412","volume":"13","author":"Laramy C. R.","year":"2019","journal-title":"ACS Nano"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201916233"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7SM00384F"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/339724a0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143826"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10867-016-9433-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200360"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsta.2000.0814"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SM01207A"},{"key":"e_1_2_8_13_1","unstructured":"Note that there was no difference in the SAXS and WAXS patterns between with or without SG (see Figure S7 in the Supporting Information)."},{"key":"e_1_2_8_14_1","unstructured":"The assembly yield can be estimated from the lattice parameters obtained (see Figure S8 in the Supporting Information)."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pol.1958.1203312618"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1088\/1367-2630\/18\/5\/055001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab1391"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2SC07063D"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-022-01234-w"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21596"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202410243","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202410243","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T15:22:26Z","timestamp":1739373746000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202410243"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,23]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/smll.202410243"],"URL":"https:\/\/doi.org\/10.1002\/smll.202410243","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,23]]},"assertion":[{"value":"2024-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2410243"}}