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For mild traumatic brain injury (mTBI), salivary miRNAs like miR\u2010let7a, miR\u201021, and miR\u201030e show promise for early detection of subtle injuries lacking reliable indicators. To advance the detection of mTBI\u2010related salivary miRNAs, this study integrates anti\u2010miRNA and miRNA hybridization\u2010based sensing with the development of a nanoscale covalent\u2010organic framework (COF) platform. COFs, with their highly customizable structures, large surface area, and biocompatibility, serve as a versatile foundation for biosensing applications. Here, post\u2010synthetic modification (PSM) of COFs is introduced for essential covalent conjugation of streptavidin for further immobilization of methylene blue\u2010labeled and biotinylated Anti\u2010miRNAs. Furthermore, the layer\u2010by\u2010layer assembly of conductive polymers enhanced the biosensor's electrical performance, enabling ultrasensitive and multiplexed detection of salivary miRNAs. 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