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Despite its unmet advantages in terms of complexity and processability in a single step, this technique is limited to the front side of the substrate. Considering the advantage of inherent topography patterns on the reverse side of the substrate, an additional degree of patterning can be envisioned. However, selective patterning on the reverse side is challenging due to the fragility of the cell culture on the front side. Herein, a technically simple scraping technique is presented in combination with 3D\u2010\u00b5CP to generate 3D cell patterns on both the front and the reverse side of a micro thermoformed substrate. The technical advancement of 3D\u2010\u00b5CP with the scraping technique offers a complex, multi\u2010layered co\u2010culture system that can be used to study cell\u2010to\u2010cell crosstalk at the microlevel with topographies similar to real tissue. Direct seeding of the third cell type on the scaffold expands cell type complexity, resulting in a tri\u2010culture model. As a proof of concept, a triculture of EA.Hy 926 endothelial cells, HepG2 hepatoblastoma cells, and NIH3T3 fibroblasts are generated, mimicking the tumor microenvironment in the liver.<\/jats:p>","DOI":"10.1002\/smll.202412409","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T19:20:29Z","timestamp":1759519229000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of 3D Micro Contact Printing and Scraping Technique for Site Selective Cocultivation Across a Porous Micro Structured Scaffold"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9445-0524","authenticated-orcid":false,"given":"Dana","family":"Brauer","sequence":"first","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anni","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Borowiec","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Hampl","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"Prehl","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maren","family":"Klett","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Merle Johanna","family":"K\u00fcstner","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Weise","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Schober","sequence":"additional","affiliation":[{"name":"Department of Nano\u2010Biosystems Engineering Institute of Chemistry and Biotechnology Ilmenau University of Technology  P.O. Box 98684 Ilmenau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6871-4294","authenticated-orcid":false,"given":"Sukhdeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Research Group Bioorganic Chemistry of Bioactive Surfaces Institute of Chemistry and Biotechnology  Prof\u2010Schmidt\u2010Stra\u00dfe 26 98693 Ilmenau Germany"},{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  P\/Bag X54001 Westville Durban 4000 South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2020.06.003"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.00033"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2016.11.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24683"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5082\/5\/4\/045008"},{"key":"e_1_2_8_6_1","first-page":"2020","volume":"8","author":"Darling N. 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