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Current research shows that these structural features have primarily been achieved using non\u2010biodegradable block copolymers, while the fundamental mechanism behind their formation is not well understood. Thus, designing biodegradable kippah vesicles and elucidating their formation mechanism is critical. In this study, a tetraphenylethylene (TPE) moiety \u2013 a luminogen with aggregation\u2010induced emission (AIE) properties \u2013 is strategically introduced into the block copolymer side chain\u2010, yielding the novel polypeptide\u00a0poly(ethylene glycol)<jats:sub>45<\/jats:sub>\u2010<jats:italic>block<\/jats:italic>\u2010poly[(glutamic acid\u2010TPE)<jats:sub>26<\/jats:sub>\u2010<jats:italic>stat<\/jats:italic>\u2010(glutamic acid)<jats:sub>29<\/jats:sub>] [PEG\u2084\u2085\u2010<jats:italic>b<\/jats:italic>\u2010P(GATPE\u2082\u2086\u2010<jats:italic>stat<\/jats:italic>\u2010GA\u2082\u2089)]. This polypeptide could self\u2010assemble into kippah vesicles driven by hydrophobic interactions and hydrogen bonding, as confirmed by Fourier\u2010transform infrared (FTIR) spectroscopy, ultraviolet\u2010visible (UV\u2013vis) absorption, photoluminescence spectroscopy, and morphological characterization across different aggregation states. Notably, the intrinsic fluorescence of these kippah vesicles exhibited high cellular internalization efficiency and excellent cytocompatibility, highlighting their potential for biomedical applications such as bioimaging and targeted cellular delivery.<\/jats:p>","DOI":"10.1002\/smll.202501838","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T02:54:18Z","timestamp":1745376858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Preparation and Mechanism Insight of Biodegradable Kippah Vesicles"],"prefix":"10.1002","volume":"21","author":[{"given":"Ping","family":"Wei","sequence":"first","affiliation":[{"name":"School of Life Sciences and Health Engineering Jiangnan University  Wuxi 214122 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Health Engineering 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