{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T22:58:44Z","timestamp":1783551524801,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"37","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["TGY24H040011"],"award-info":[{"award-number":["TGY24H040011"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Small"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Preeclampsia in pregnancies affects angiogenesis while the fetus develops. Early diagnosis of preeclampsia can enable timely prognosis and intervention. This study developed a smartphone\u2010based, label\u2010free dual\u2010channel electrochemical biosensing platform for the simultaneous detection of preeclampsia biomarkers, placental growth factor (PLGF) and soluble Fms\u2010like tyrosine kinase\u20101 (sFlt\u20101). Based on the functional modification of gold nanoparticles and reduced graphene oxide on working electrodes, the specific surface area is enlarged, and the stability is improved. PLGF and sFlt\u20101 antibody serving as sensitive elements, are immobilized on the electrode surface. As PLGF binds sFlt\u20101 dynamically, fluidic chambers are designed based on 3D printing for comprehensive detection. The biosensor achieved linear ranges of 10\u20138000 pg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for sFlt\u20101 and 1\u2013250 pg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for PLGF, meeting clinical diagnostic requirements. Molecular docking is used to analyze the binding properties and signaling pathways of PLGF and sFlt\u20101, providing critical diagnostic insights for clinicians. This biosensing platform, featuring a portable integrated circuit and smartphone\u2010based APP, enables point\u2010of\u2010care testing (POCT). Comparison tests using plasma samples from 10 individuals demonstrated a strong correlation (r &gt; 0.98,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a00.1) with ELISA, indicating the potential in clinical POCT applications for preeclampsia. These results suggest that the sensor can be used as a dual\u2010channel biomarker for preeclampsia without sample labeling.\n                  <\/jats:p>","DOI":"10.1002\/smll.202503452","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T05:29:26Z","timestamp":1754458166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Smartphone Dual\u2010Channel Biosensor for Simultaneous PLGF\/sFlt\u20101 Detection and Signaling Pathway Analysis"],"prefix":"10.1002","volume":"21","author":[{"given":"Zetao","family":"Chen","sequence":"first","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixia","family":"Li","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanlong","family":"Li","sequence":"additional","affiliation":[{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songsong","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"He","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqiong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology the Third Clinical Institute Affiliated to Wenzhou Medical University  Wenzhou 325035 China"},{"name":"The Third Affiliated Hospital of Shanghai University  Shanghai 201999 China"},{"name":"Wenzhou People's Hospital Wenzhou Maternal and Child Health Care Hospital  Wenzhou 325000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology the Third Clinical Institute Affiliated to Wenzhou Medical University  Wenzhou 325035 China"},{"name":"The Third Affiliated Hospital of Shanghai University  Shanghai 201999 China"},{"name":"Wenzhou People's Hospital Wenzhou Maternal and Child Health Care Hospital  Wenzhou 325000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health School of Public Health Tianjin Medical University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cha","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Tianjin Medical University General Hospital  Tianjin 300072 China"},{"name":"Tianjin Key Laboratory of Female Reproductive Health and Eugenics Tianjin Medical University General Hospital  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Chen","sequence":"additional","affiliation":[{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Lin","sequence":"additional","affiliation":[{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Li","sequence":"additional","affiliation":[{"name":"Medical School of Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Safety Engineering Tianjin University of Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furong","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health Sciences School of Public Health Peking University  Beijing 100000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shike","family":"Hou","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1141-9646","authenticated-orcid":false,"given":"Liqiong","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Disaster and Emergency Medicine Tianjin University  Tianjin 300072 China"},{"name":"Wenzhou Safety (Emergency) Institute Tianjin University  Wenzhou 325000 China"},{"name":"Tianjin Key Laboratory of Disaster Medicine Technology  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/jalm\/jfad003"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S297344"},{"key":"e_1_2_9_3_1","first-page":"49, 211","author":"Xue S. Y.","year":"2022","journal-title":"Clin. Exp. Obstet. Gynecol."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.15338"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.13715"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.26032"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.121.17258"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.14974"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2022.04.015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2022.123592"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113649"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113572"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1765"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2020.116159"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2021.1960792"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/INMD.20220009"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111875"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112724"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114994"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112217"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-019-01647-z"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios15030140"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202206461"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130447"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2020.128825"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.113204"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-018-4979-4"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12905-018-0677-y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115447"},{"key":"e_1_2_9_30_1","first-page":"113","volume":"9","author":"Zarrin R.","year":"2022","journal-title":"Anal. Bioanal. Chem. Res."},{"key":"e_1_2_9_31_1","first-page":"1881","volume":"104","author":"Shalvi S.","year":"2022","journal-title":"Int. J. Environ. Anal. Chem."},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2018.068"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111694"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113914"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313237200"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641963.2020.1756313"},{"key":"e_1_2_9_37_1","first-page":"428","volume":"17","author":"Liu Y.","year":"2016","journal-title":"Chin. J. Clin. Obstet. Gynecol."},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.19070"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.20361"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01661"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2017.1291673"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.13763"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.24874"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641955.2022.2066119"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.22093"},{"key":"e_1_2_9_46_1","first-page":"259","author":"Du H.","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"e_1_2_9_47_1","first-page":"43, 153","author":"G\u00f6hrig A.","year":"2024","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.18240\/ijo.2023.09.16"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.202503452","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T14:21:31Z","timestamp":1763734891000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.202503452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,5]]},"references-count":48,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/smll.202503452"],"URL":"https:\/\/doi.org\/10.1002\/smll.202503452","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,5]]},"assertion":[{"value":"2025-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03452"}}