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Herein, a nanobody\u2010based trispecific T cell nanoengagers platform, termed Tri\u2010Nb@FNP is introduced, which leverages the modular and programmable assembly of nanobodies on ferritin nanoparticles (FNP) via SpyTag\/SpyCatcher bioconjugation technology. These nanoengagers are designed to target EGFR\u2010positive triple\u2010negative breast cancer (TNBC) or HER2 positive breast cancer, alongside CD3 and CD28 on T cells, thereby facilitating precise immune synapse formation. This synergistic interaction robustly enhances T cell activation, proliferation and cytotoxicity, promotes cytotoxic CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell infiltration into the tumor microenvironment, and achieves significant tumor suppression both in vitro and in vivo. Tri\u2010Nb@FNP is thus established as a novel class of Tri\u2010TCEs, offering a simple and efficient self\u2010assembly platform to integrate multispecific targeting molecules for advancing cancer immunotherapy.\n                  <\/jats:p>","DOI":"10.1002\/smll.202504869","type":"journal-article","created":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T19:26:18Z","timestamp":1760901978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Self\u2010Assembled Trispecific T Cell Nanoengagers for Enhanced Cancer Immunotherapy"],"prefix":"10.1002","volume":"21","author":[{"given":"Guixi","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences Faculty of Medicine Tianjin University  Tianjin 300072 China"},{"name":"Zhejiang Cancer Hospital The Key Laboratory of Zhejiang Province for Basic and Clinical Application of Functional Nucleic Acids Hangzhou Institute of Medicine (HIM) Chinese 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