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Here, 3D Single\u2010Molecule Active\u2010feedback Real\u2010time Tracking (3D\u2010SMART) microscopy is used to resolve nanoparticle dynamics in agarose gels with unprecedented spatiotemporal resolution. This highlights \u201chopping diffusion\u201d, where particles intermittently escape confinement pockets, providing insights into hydrogel microstructure. Long, highly sampled trajectories enable the extraction of kinetic parameters, confinement sizes, and thermodynamic barriers. This study demonstrates 3D\u2010SMART's ability to probe particle\u2010environment interactions at super\u2010resolution (\u224810\u00a0nm in XY and \u224830\u00a0nm in Z) in 3D, offering new perspectives on nanoparticle diffusion and the structural dynamics of porous materials, with implications for drug delivery, material science, and biological systems.<\/jats:p>","DOI":"10.1002\/smll.202505319","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T06:39:41Z","timestamp":1755326381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Super\u2010Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High\u2010Speed 3D Active\u2010Feedback Single\u2010Particle Tracking 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