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Here, inspired by the intricate architecture of hepatic lobules, a novel biomimetic vascularized liver\u2010on\u2010a\u2010chip fabricated via high\u2010precision 3D printing technology is presented. The chip features a capillary network with multiple micro\u2010pores, ensuring a constant nutrient supply and efficient substance exchange within the cell environment through microfluidic perfusion. Under microperfusion conditions, human induced pluripotent stem cell\u2010derived hepatocytes (hiPSC\u2010Heps) exhibit superior physiological status and biological functionality compared to traditional 2D cultures. In addition, a comprehensive platform for assessing drug\u2010induced liver injury (DILI) is established by integrating a concentration gradient chip, liver chips, and multicellular coculture technologies. This innovative platform effectively validates both acute and chronic hepatotoxic effects of acetaminophen (APAP). These findings demonstrate that the biomimetic vascularized liver\u2010on\u2010a\u2010chip exhibits ideal physiological relevance and holds significant potential for applications in drug development and toxicity screening.<\/jats:p>","DOI":"10.1002\/smll.202505653","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:12:34Z","timestamp":1760425954000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Microperfused Biomimetic Liver\u2010on\u2010a\u2010Chip for High\u2010Throughput Hepatotoxicity Screening"],"prefix":"10.1002","volume":"21","author":[{"given":"Junqi","family":"Zhao","sequence":"first","affiliation":[{"name":"Joint Centre of Translational Medicine Wenzhou Institute of University of Chinese Academy of Sciences The First Affiliated Hospital of Wenzhou Medical University  Wenzhou 325035 P. R. 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