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However, many existing systems struggle with achieving precise control over the release timing. Here, a photothermal trigger\u2010responsive delivery platform based on Giant Unilamellar Vesicles (GUVs) functionalized with gold nanorods (GNRs) is described to overcome these challenges. Toward this end, cargo\u2010loaded GUVs are produced using the emulsion transfer method and effective GNR attachment to the vesicle membrane is achieved by functionalizing the GNRs with cholesterol. The near\u2010infrared light (NIR)\u2010mediated cargo release mechanism is described. Importantly, high release efficiency is achieved by optimizing the cholesterol concentration on the GNRs, which is essential for controlling the GNR attachment mechanism to the GUV membrane. Finally, GNR\u2010functionalized ampicillin\u2010loaded GUVs are tested in the presence of\n                    <jats:italic>E. coli<\/jats:italic>\n                    bacteria to demonstrate the platform's functionality. Following NIR\u2010triggered antibiotic release, bacterial growth is inhibited. This showcases the practical potential of the developed trigger\u2010responsive system. Notably, GNR\u2010mediated photothermal heating alone also reduces bacterial viability upon extended illumination, indicating a dual antibacterial mechanism. This versatile and biocompatible system offers a controlled delivery method with broad applicability for therapeutics, diagnostics, and other biomedical applications.\n                  <\/jats:p>","DOI":"10.1002\/smll.202505852","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T14:01:07Z","timestamp":1762956067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design of a Trigger\u2010Responsive Photothermal Vesicle\u2010Based Cargo Delivery Platform"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2439-9228","authenticated-orcid":false,"given":"Anastassiya","family":"Schramm","sequence":"first","affiliation":[{"name":"Department of Cellular Biophysics Max Planck Institute for Medical Research  Jahnstra\u00dfe 29 69120 Heidelberg Germany"},{"name":"Max Planck School Matter to Life  Jahnstra\u00dfe 29 69120 Heidelberg 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